• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Septin 10 Polyclonal antibody

Septin 10 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12420-1-AP

Synonyms

SEPT10, septin 10, septin10

Validation Data Gallery

Applications:
  • WB analysis of human kidney using 12420-1-AP

    WB analysis of human kidney using 12420-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12420-1-AP (Septin 10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 12420-1-AP

    WB analysis of HeLa using 12420-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12420-1-AP (Septin 10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 12420-1-AP

    IHC staining of human pancreas cancer using 12420-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12420-1-AP (Septin 10 antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HepG2 using 12420-1-AP

    IF Staining of HepG2 using 12420-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12420-1-AP (Septin 10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman kidney tissue, HeLa cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12420-1-AP targets Septin 10 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Septin 10 fusion protein Ag3071 相同性解析による交差性が予測される生物種
Full Name septin 10
Calculated molecular weight 53 kDa, 57 kDa
Observed molecular weight 53-57 kDa
GenBank accession numberBC020502
Gene symbol SEPT10
Gene ID (NCBI) 151011
RRIDAB_2186992
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Septin 10 antibody 12420-1-APDownload protocol
IHC protocol for Septin 10 antibody 12420-1-APDownload protocol
IF protocol for Septin 10 antibody 12420-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols