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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using SENP5-Specific Polyclonal antibody (19529-1-AP)

    WB analysis of mouse liver using 19529-1-AP (same clone as 19529-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 19529-1-AP (SENP5-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19529-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SENP5-Specific Polyclonal antibody (19529-1-AP)

    WB analysis of HepG2 using 19529-1-AP (same clone as 19529-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19529-1-AP (SENP5-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19529-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using SENP5-Specific Polyclonal antibody (19529-1-AP)

    IP experiment of HeLa using 19529-1-AP (same clone as 19529-1-PBS)

    IP result of anti-SENP5-Specific (IP:19529-1-AP, 4ug; Detection:19529-1-AP 1:300) with HeLa cells lysate 520ug. This data was developed using the same antibody clone with 19529-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SENP5-Specific Polyclonal antibody (19529-1-AP)

    IHC staining of human cervical cancer using 19529-1-AP (same clone as 19529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19529-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SENP5-Specific Polyclonal antibody (19529-1-AP)

    IHC staining of human cervical cancer using 19529-1-AP (same clone as 19529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19529-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SENP5-Specific Polyclonal antibody (19529-1-AP)

    IHC staining of human placenta using 19529-1-AP (same clone as 19529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19529-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SENP5-Specific Polyclonal antibody (19529-1-AP)

    IHC staining of human placenta using 19529-1-AP (same clone as 19529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19529-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SENP5-Specific Polyclonal antibody (19529-1-AP)

    IHC staining of human cervical cancer using 19529-1-AP (same clone as 19529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19529-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SENP5-Specific Polyclonal antibody (19529-1-AP)

    IHC staining of human cervical cancer using 19529-1-AP (same clone as 19529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19529-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SENP5-Specific Polyclonal antibody, PBS Only

SENP5-Specific Polyclonal Antibody for IHC, IP, Indirect ELISA
Cat No. 19529-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, Indirect ELISA

Synonyms

SENP5, Sentrin-specific protease 5, Sentrin/SUMO-specific protease SENP5, EC:3.4.22.-

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19529-1-PBS targets SENP5-Specific in IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name SUMO1/sentrin specific peptidase 5
Calculated molecular weight 87 kDa
Observed molecular weight 97 kDa
GenBank accession numberNM_152699
Gene Symbol SENP5
Gene ID (NCBI) 205564
RRIDAB_10643377
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96HI0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SENP5, also named as FKSG45, belongs to the peptidase C48 family. SENP5 is a protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. SENP5 has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. It is required for cell division. The antibody is specific to SENP5.

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