Validation Data Gallery
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Tested Applications
| Positive WB detected in | Y79 cells, NIH/3T3 cells, HeLa cells, HepG2 cells, Caco-2 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85837-3-RR targets SENP3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | SENP3 fusion protein Ag11911 相同性解析による交差性が予測される生物種 |
| Full Name | SUMO1/sentrin/SMT3 specific peptidase 3 |
| Calculated molecular weight | 574 aa, 65 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC080658 |
| Gene Symbol | SENP3 |
| Gene ID (NCBI) | 26168 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H4L4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SENP3(Sentrin-specific protease 3) is also named as SSP3, SUSP3 and belongs to the peptidase C48 family. It acts as an essential factor for ribosome biogenesis. SENP3 might regulate the sumoylation of other substrates through directly binding or mediating association with NPM1 at the nucleolus and other subcellular compartments.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SENP3 antibody 85837-3-RR | Download protocol |
| IF protocol for SENP3 antibody 85837-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |