Validation Data Gallery
Tested Applications
| Positive WB detected in | EC109 cells, MKN-45 cells, SGC-7901 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
29772-1-AP targets SENP2 in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30728 Product name: Recombinant human SENP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 78-228 aa of NM_021627 Sequence: TKPMVTSACNGTRNVAPSGEVFSNSSSCELTGSGSWNNMLKLGNKSPNGISDYPKIRVTVTRDQPRRVLPSFGFTLNSEGCNRRPGGRRHSKGNPESSLMWKPQEQAVTEMISEESGKGLRRPHCTVEEGVQKEEREKYRKLLERLKESGH 相同性解析による交差性が予測される生物種 |
| Full Name | SUMO1/sentrin/SMT3 specific peptidase 2 |
| Calculated molecular weight | 68 kDa |
| Observed molecular weight | 60-68 kDa |
| GenBank accession number | NM_021627 |
| Gene Symbol | SENP2 |
| Gene ID (NCBI) | 59343 |
| RRID | AB_3086158 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HC62 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SENP2 (Sentrin-specific protease 2), as a protease, catalyzes two essential functions in the SUMO pathway. The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins (PMID:15296745). The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein (PMID:20194620, PMID:21965678). It has 2 isoforms with the molecular weight of 66 and 68 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SENP2 antibody 29772-1-AP | Download protocol |
| WB protocol for SENP2 antibody 29772-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |