• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SEMA6B Polyclonal antibody (22571-1-AP)

    WB analysis using 22571-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22571-1-AP (SEMA6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SEMA6B Polyclonal antibody (22571-1-AP)

    IP experiment of mouse brain using 22571-1-AP

    IP result of anti-SEMA6B (IP:22571-1-AP, 4ug; Detection:22571-1-AP 1:1000) with mouse brain tissue lysate 1120 ug.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

SEMA6B Polyclonal antibody

SEMA6B Polyclonal Antibody for WB, IP, ELISA
Cat No. 22571-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

SEM SEMA Y, SEMA VIB, Sema Z, SEMAN, Semaphorin 6B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, MDA-MB-231 cells, mouse brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22571-1-AP targets SEMA6B in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEMA6B fusion protein Ag16707 相同性解析による交差性が予測される生物種
Full Name sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B
Calculated molecular weight95, 74 kDa
Observed molecular weight70 kDa
GenBank accession numberBC142617
Gene Symbol SEMA6B
Gene ID (NCBI) 10501
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9H3T3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Semaphorins (SEMAs) play critical roles in inflammation, angiogenesis, apoptosis, fibrosis, bone remodeling, and cell invasion. SEMA6B, the sixth member of the SEMA family, has shown significant correlations with the stage of liver fibrosis in hepatitis C virus-infected patients. SEMA6B is also involved in inflammatory responses and serves as a biomarker for macrophage-specific genes. Additionally, the level of SEMA6B in circulating monocytes has predictive value for cardiovascular disease. Also, SEMA6B can be a valuable biomarker for diagnosing HBV-ACLF12 (PMID: 39267780).

Protocols

Product Specific Protocols
WB protocol for SEMA6B antibody 22571-1-APDownload protocol
IP protocol for SEMA6B antibody 22571-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols