Validation Data Gallery
Tested Applications
| Positive WB detected in | COLO 320 cells, HeLa cells, SH-SY5Y cells, SW480 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12279-1-AP targets SEMA4G in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2928 Product name: Recombinant human SEMA4G protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-127 aa of BC020960 Sequence: MGSMSPPSAWPCVLDGPETRQDLCQPPKPCVHSHAHMEECLSAGLQCPHPHLLLVHSCFIPASGLGVPSQLPHPIWSSSPAPCGDLFVKSLGTGQPGEVRLHHSPPLPSCVALVNQPPHSPWSFSRV 相同性解析による交差性が予測される生物種 |
| Full Name | sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G |
| Calculated molecular weight | 91 kDa |
| Observed molecular weight | 105 kDa |
| GenBank accession number | BC020960 |
| Gene Symbol | SEMA4G |
| Gene ID (NCBI) | 57715 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NTN9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Semaphorins constitute a large and growing gene family, several members of which are axon guidance molecules. SEMA4G, a novel class IV member of the semaphorin gene family, located on mouse chromosome 19. SEMA4G is expressed early in development in the brain, spinal cord, and several sensory organs as well as specific populations of projection neurons, compatible with the well-established function of semaphorins as axon guidance molecules.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SEMA4G antibody 12279-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |