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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using SEMA4F Polyclonal antibody (12474-1-AP)

    WB analysis of mouse lung using 12474-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using SEMA4F Polyclonal antibody (12474-1-AP)

    WB analysis of Y79 using 12474-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SEMA4F Polyclonal antibody (12474-1-AP)

    WB analysis of mouse brain using 12474-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SEMA4F Polyclonal antibody (12474-1-AP)

    WB analysis of mouse brain using 12474-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SEMA4F Polyclonal antibody (12474-1-AP)

    WB analysis of HepG2 using 12474-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using SEMA4F Polyclonal antibody (12474-1-AP)

    WB analysis of human liver using 12474-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12474-1-AP (SEMA4F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using SEMA4F Polyclonal antibody (12474-1-AP)

    IP experiment of HepG2 using 12474-1-AP

    IP result of anti-SEMA4F (IP:12474-1-AP, 4ug; Detection:12474-1-AP 1:500) with HepG2 cells lysate 3600ug.

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SEMA4F Polyclonal antibody

SEMA4F Polyclonal Antibody for WB, IP, ELISA
Cat No. 12474-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

M SEMA, m Sema M, PRO2353, Sema M, Sema W, SEMA4F, SEMAM, Semaphorin 4F, Semaphorin M, Semaphorin W, SEMAW

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse lung tissue, HepG2 cells, human liver tissue, mouse brain tissue, Y79 cells
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12474-1-AP targets SEMA4F in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEMA4F fusion protein Ag3082 相同性解析による交差性が予測される生物種
Full Name sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4F
Calculated molecular weight 615 aa, 66 kDa
Observed molecular weight 66 kDa, 84 kDa
GenBank accession numberBC018361
Gene Symbol SEMA4F
Gene ID (NCBI) 10505
RRIDAB_2185847
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95754
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SEMA4F antibody 12474-1-APDownload protocol
IP protocol for SEMA4F antibody 12474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols