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Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse SEMA4A Rabbit Recombinant Antibody (98513-2-RR)

    FC experiment of mouse splenocytes using 98513-2-RR (same clone as 98513-2-PBS)

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse SEMA4A Rabbit RecAb (98513-2-RR, Clone: 250325B12) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 488 Anti-Mouse CD3. Cells were not fixed. This data was developed using the same antibody clone with 98513-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse SEMA4A Rabbit Recombinant Antibody (98513-2-RR)

    FC experiment of mouse splenocytes using 98513-2-RR (same clone as 98513-2-PBS)

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse SEMA4A Rabbit RecAb (98513-2-RR, Clone: 250325B12) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98513-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using Anti-Mouse SEMA4A Rabbit Recombinant Antibody (98513-2-RR)

    FC experiment of Transfected HEK-293T using 98513-2-RR (same clone as 98513-2-PBS)

    1x10^6 HEK-293T cells or SEMA4A transfected HEK-293T cells were surface stained with 0.25 ug Anti-Mouse Sema4a Rabbit RecAb (98513-2-RR, Clone: 250325B12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98513-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using Anti-Mouse SEMA4A Rabbit Recombinant Antibody (98513-2-RR)

    FC experiment of Transfected HEK-293T using 98513-2-RR (same clone as 98513-2-PBS)

    1x10^6 HEK-293T cells (left) or SEMA4A transfected HEK-293T cells (right) were surface stained with 0.25 ug Anti-Mouse SEMA4A Rabbit RecAb (98513-2-RR, Clone: 250325B12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98513-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98513-2-RR

    Affinity and Kinetic Characterization of 98513-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98513-2-RR against Mouse SEMA4A were performed. The affinity constant is below 1 pM.

Anti-Mouse SEMA4A Rabbit Recombinant Antibody, PBS Only

SEMA4A Uni-rAb® Recombinant Antibody for FC
Cat No. 98513-2-PBS
Clone No.250325B12

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Sema B, Semab, Semaphorin-4A, Semaphorin-B, SemB

Formulation:  PBS Only
PBS, Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98513-2-PBS targets SEMA4A in FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg2680

Product name: Recombinant Mouse SEMA4A protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 33-682 aa of NM_001163489.1

Sequence: TGGQGPMPRVKYHAGDGHRALSFFQQKGLRDFDTLLLSDDGNTLYVGAREAVLALNIQNPGIPRLKNMIPWPASERKKTECAFKKKSNETQCFNFIRVLVSYNATHLYACGTFAFSPACTFIELQDSLLLPILIDKVMDGKGQSPFDPVHKHTAVLVDGMLYSGTMNNFLGSEPILMRTLGSQPVLKTDIFLRWLHADASFVAAIPSTQVVYFFFEETASEFDFFEELYISRVAQVCKNDVGGEKLLQKKWTTFLKAQLLCAQPGQLPFNIIRHAVLLPADSPSVSRIYAVFTSQWQVGGTRSSAVCAFSLTDIERVFKGKYKELNKETSRWTTYRGSEVSPRPGSCSMGPSSDKALTFMKDHFLMDEHVVGTPLLVKSGVEYTRLAVESARGLDGSSHVVMYLGTSTGSLHKAVVPQDSSAYLVEEIQLSPDSEPVRNLQLAPAQGAVFAGFSGGIWRVPRANCSVYESCVDCVLARDPHCAWDPESRLCSLLSGSTKPWKQDMERGNPEWVCTRGPMARSPRRQSPPQLIKEVLTVPNSILELPCPHLSALASYHWSHGRAKISEASATVYNGSLLLLPQDGVGGLYQCVATENGYSYPVVSYWVDSQDQPLALDPELAGVPRERVQVPLTRVGGGASMAAQRSYWPH

相同性解析による交差性が予測される生物種
Full Name sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A
Calculated molecular weight83 kDa
GenBank accession numberNM_001163489.1
Gene Symbol Sema4a
Gene ID (NCBI) 20351
RRIDAB_3746143
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ62178
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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