Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30638-1-AP targets SEMA3C in IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32426 Product name: Recombinant human SEMA3C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 560-751 aa of BC030690 Sequence: PLTQCRGFNLKAYRNAAEIVQYGVKNNTTFLECAPKSPQASIKWLLQKDKDRRKEVKLNERIIATSQGLLIRSVQGSDQGLYHCIATENSFKQTIAKINFKVLDSEMVAVVTDKWSPWTWASSVRALPFHPKDIMGAFSHSEMQMINQYCKDTRQQHQQGDESQKMRGDYGKLKALINSRKSRNRRNQLPES 相同性解析による交差性が予測される生物種 |
| Full Name | sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C |
| Calculated molecular weight | 85 kDa |
| GenBank accession number | BC030690 |
| Gene Symbol | SEMA3C |
| Gene ID (NCBI) | 10512 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99985 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SEMA3C (Semaphorin 3C) is associated with tumor progression and poor prognosis across multiple tumor types, including pancreatic, gastric, prostate, and breast cancer, as well as glioma. It is worth noting that full-length SEMA3C has also been reported as a tumor suppressor factor by suppressing tumor lymphangiogenesis and metastasis (PMID: 31649890).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SEMA3C antibody 30638-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |