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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEH1L Polyclonal antibody (15565-1-AP)

    WB analysis using 15565-1-AP (same clone as 15565-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15565-1-AP (SEH1L antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15565-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SEH1L Polyclonal antibody (15565-1-AP)

    WB analysis using 15565-1-AP (same clone as 15565-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15565-1-AP (SEH1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15565-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using SEH1L Polyclonal antibody (15565-1-AP)

    IF Staining of A549 using 15565-1-AP (same clone as 15565-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SEH1L antibody (15565-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 15565-1-PBS in a different storage buffer formulation.

SEH1L Polyclonal antibody, PBS Only

SEH1L Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 15565-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

SEH1, SEC13-like protein, SEC13L, Nup107-160 subcomplex subunit SEH1, Nucleoporin SEH1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15565-1-PBS targets SEH1L in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7795

Product name: Recombinant human SEH1L protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-360 aa of BC012430

Sequence: MFVARSIAADHKDLIHDVSFDFHGRRMATCSSDQSVKVWDKSESGDWHCTASWKTHSGSVWRVTWAHPEFGQVLASCSFDRTAAVWEEIVGESNDKLRGQSHWVKRTTLVDSRTSVTDVKFAPKHMGLMLATCSADGIVRIYEAPDVMNLSQWSLQHEISCKLSCSCISWNPSSSRAHSPMIAVGSDDSSPNAMAKVQIFEYNENTRKYAKAETLMTVTDPVHDIAFAPNLGRSFHILAIATKDVRIFTLKPVRKELTSSGGPTKFEIHIVAQFDNHNSQVWRVSWNITGTVLASSGDDGCVRLWKANYMDNWKCTGILKGNGSPVNGSSQQGTSNPSLGSTIPSLQNSLNGSSAGRKHS

相同性解析による交差性が予測される生物種
Full Name SEH1-like (S. cerevisiae)
Calculated molecular weight 39.6 kDa
Observed molecular weight36-39 kDa
GenBank accession numberBC012430
Gene Symbol SEH1L
Gene ID (NCBI) 81929
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96EE3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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