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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated SECTM1 Monoclonal antibody

SECTM1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C11B4

Cat no : CL488-60281

Synonyms

K12, Protein K 12, secreted and transmembrane 1, SECTM1

Validation Data Gallery

Applications:
  • FC experiment of PC-3 using CL488-60281

    FC experiment of PC-3 using CL488-60281

    1X10^6 PC-3 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human SECTM1 (CL488-60281, Clone:1C11B4) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inPC-3 cells
For other applications, we recommend the unconjugated version of this antibody, 60281-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-60281 targets SECTM1 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen SECTM1 fusion protein Ag14645 相同性解析による交差性が予測される生物種
Full Name secreted and transmembrane 1
Calculated molecular weight 248 aa, 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC017716
Gene symbol SECTM1
Gene ID (NCBI) 6398
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Thiophilic affinity chromatograph
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SECTM1, also named as protein K12, is originally identified by its location just upstream of the CD7 locus. It has been proposed as a ligand for CD7. SECTM1 is involved in thymocyte signaling. This antibody is specific to SECTM1.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 SECTM1 antibody CL488-60281Download protocol
Standard Protocols
Click here to view our Standard Protocols