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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SECTM1 Monoclonal antibody, PBS Only

SECTM1 Monoclonal Antibody for WB, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C11B4

Cat no : 60281-1-PBS

Synonyms

K12, Protein K 12, secreted and transmembrane 1, SECTM1

Validation Data Gallery

Applications:
  • WB analysis using 60281-1-Ig (same clone as 60281-1-PBS)

    WB analysis using 60281-1-Ig (same clone as 60281-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60281-1-Ig (SECTM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60281-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60281-1-Ig (same clone as 60281-1-PBS)

    IHC staining of human breast cancer using 60281-1-Ig (same clone as 60281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60281-1-Ig (SECTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60281-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60281-1-Ig (same clone as 60281-1-PBS)

    IHC staining of human breast cancer using 60281-1-Ig (same clone as 60281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60281-1-Ig (SECTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60281-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 60281-1-Ig (same clone as 60281-1-PBS)

    IHC staining of human colon cancer using 60281-1-Ig (same clone as 60281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60281-1-Ig (SECTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60281-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 60281-1-Ig (same clone as 60281-1-PBS)

    FC experiment of K-562 using 60281-1-Ig (same clone as 60281-1-PBS)

    1X10^6 K-562 cells were stained with 0.2ug SECTM1 antibody (60281-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. This data was developed using the same antibody clone with 60281-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

60281-1-PBS targets SECTM1 in WB, FC, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen SECTM1 fusion protein Ag14645 相同性解析による交差性が予測される生物種
Full Name secreted and transmembrane 1
Calculated molecular weight 248 aa, 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC017716
Gene symbol SECTM1
Gene ID (NCBI) 6398
Conjugate Unconjugated
Form Liquid
Purification Method Thiophilic affinity chromatograph
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SECTM1, also named as protein K12, is originally identified by its location just upstream of the CD7 locus. It has been proposed as a ligand for CD7. SECTM1 is involved in thymocyte signaling. This antibody is specific to SECTM1.