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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEC63 Polyclonal antibody (28665-1-AP)

    WB analysis using 28665-1-AP (same clone as 28665-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28665-1-AP (SEC63 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SEC63 Polyclonal antibody (28665-1-AP)

    WB analysis of HepG2 using 28665-1-AP (same clone as 28665-1-PBS)

    WB result of SEC63 antibody (28665-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEC63 transfected HepG2 cells. This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SEC63 Polyclonal antibody (28665-1-AP)

    IHC staining of mouse pancreas using 28665-1-AP (same clone as 28665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28665-1-AP (SEC63 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SEC63 Polyclonal antibody (28665-1-AP)

    IHC staining of mouse pancreas using 28665-1-AP (same clone as 28665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28665-1-AP (SEC63 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SEC63 Polyclonal antibody (28665-1-AP)

    IHC staining of human liver using 28665-1-AP (same clone as 28665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28665-1-AP (SEC63 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using SEC63 Polyclonal antibody (28665-1-AP)

    IHC staining of human liver using 28665-1-AP (same clone as 28665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28665-1-AP (SEC63 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SEC63 Polyclonal antibody (28665-1-AP)

    IF Staining of HEK-293 using 28665-1-AP (same clone as 28665-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SEC63 antibody (28665-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28665-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SEC63 Polyclonal antibody, PBS Only

SEC63 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28665-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DnaJ homolog subfamily C member 23, DNAJC23, SEC63L, Translocation protein SEC63 homolog

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28665-1-PBS targets SEC63 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30213

Product name: Recombinant human SEC63 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 559-728 aa of BC047221

Sequence: EAAVKEDEEEVSDKGSDSEEEETNRDSQSEKDDGSDRDSDREQDEKQNKDDEAEWQELQQSIQRKERALLETKSKITHPVYSLYFPEEKQEWWWLYIADRKEQTLISMPYHVCTLKDTEEVELKFPAPGKPGNYQYTVFLRSDSYMGLDQIKPLKLEVHEAKPVPENHPQ

相同性解析による交差性が予測される生物種
Full Name SEC63 homolog (S. cerevisiae)
Calculated molecular weight 88 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC047221
Gene Symbol SEC63
Gene ID (NCBI) 11231
RRIDAB_2881189
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGP8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SEC63 is a integral ER membrane protein. SEC63 and SEC62 form a dimeric complex and play a central role in translocation of nascent and newly synthesized precursor polypeptides into the ER. Mutations in the gene encoding SEC63 cause polycystic liver disease in humans. The intact SEC63 protein is usually detected around 83-90 kDa, while smaller cleavage products can be observed by autophagy proteolysis (PMID: 25052096)

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