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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SEC61B-Specific Polyclonal antibody

SEC61B-Specific Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14846-1-AP

Synonyms

Sec61 beta subunit, SEC61B, SEC61B-Specific

Validation Data Gallery

Applications:
  • WB analysis using 14846-1-AP

    WB analysis using 14846-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 14846-1-AP

    WB analysis of HeLa using 14846-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human kidney using 14846-1-AP

    WB analysis of human kidney using 14846-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 14846-1-AP

    IHC staining of mouse liver using 14846-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 14846-1-AP

    IHC staining of mouse liver using 14846-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 14846-1-AP

    IHC staining of human cervical cancer using 14846-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14846-1-AP (SEC61B-Specific Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human cervical cancer using 14846-1-AP

    IHC staining of human cervical cancer using 14846-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14846-1-AP (SEC61B-Specific Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human liver using 14846-1-AP

    IHC staining of human liver using 14846-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14846-1-AP (SEC61B-Specific Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 14846-1-AP

    IHC staining of human liver using 14846-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14846-1-AP (SEC61B-Specific Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of mouse pancreas using 14846-1-AP

    IF Staining of mouse pancreas using 14846-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B-Specific antibody (14846-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse pancreas using 14846-1-AP

    IF Staining of mouse pancreas using 14846-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B-Specific antibody (14846-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 14846-1-AP

    IF Staining of HeLa using 14846-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SEC61B-Specific antibody (14846-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, human kidney tissue, mouse kidney tissue, mouse liver tissue, rat kidney tissue
Positive IHC detected inmouse liver tissue, human cervical cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse pancreas tissue, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

14846-1-AP targets SEC61B-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Sec61 beta subunit
Calculated molecular weight 10 kDa
Observed molecular weight 10-15 kDa
GenBank accession numberNM_006808
Gene symbol SEC61B
Gene ID (NCBI) 10952
RRIDAB_2270168
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).

Protocols

Product Specific Protocols
WB protocol for SEC61B-Specific antibody 14846-1-APDownload protocol
IHC protocol for SEC61B-Specific antibody 14846-1-APDownload protocol
IF protocol for SEC61B-Specific antibody 14846-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Pathog

Legionella hijacks the host Golgi-to-ER retrograde pathway for the association of Legionella-containing vacuole with the ER.

Authors - Mio Kawabata
View Article