• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SEC61B Recombinant monoclonal antibody (85805-2-RR)

    WB analysis using 85805-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85805-2-RR (SEC61B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SEC61B Recombinant monoclonal antibody (85805-2-RR)

    WB analysis of HepG2 using 85805-2-RR

    WB result of SEC61B antibody (85805-2-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEC61B transfected HepG2 cells.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SEC61B Recombinant monoclonal antibody (85805-2-RR)

    IF Staining of HeLa using 85805-2-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SEC61B antibody (85805-2-RR, Clone: 250082D3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 85805-2-RR

    Affinity of 85805-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85805-2-RR against Human SEC61B were performed. The affinity constant is 28.9 pM.

  • KD/KO Validated

SEC61B Recombinant monoclonal antibody

SEC61B Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85805-2-RR
Clone No.250082D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Protein transport protein Sec61 subunit beta

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293T cells, A431 cells, L02 cells, NIH/3T3 cells, C6 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85805-2-RR targets SEC61B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag7169

Product name: Recombinant human SEC61B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-96 aa of BC001734

Sequence: MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGRTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS

相同性解析による交差性が予測される生物種
Full Name Sec61 beta subunit
Calculated molecular weight 10 kDa
Observed molecular weight 10-15 kDa
GenBank accession numberBC001734
Gene Symbol SEC61B
Gene ID (NCBI) 10952
RRIDAB_3744390
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP60468
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).

Protocols

Product Specific Protocols
IF protocol for SEC61B antibody 85805-2-RRDownload protocol
WB protocol for SEC61B antibody 85805-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...