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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEC61B Polyclonal antibody (51020-2-AP)

    WB analysis using 51020-2-AP (same clone as 51020-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 51020-2-AP (SEC61B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SEC61B Polyclonal antibody (51020-2-AP)

    IHC staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SEC61B Polyclonal antibody (51020-2-AP)

    IHC staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SEC61B Polyclonal antibody (51020-2-AP)

    IHC staining of mouse liver using 51020-2-AP (same clone as 51020-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SEC61B Polyclonal antibody (51020-2-AP)

    IHC staining of mouse liver using 51020-2-AP (same clone as 51020-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using SEC61B Polyclonal antibody (51020-2-AP)

    IF Staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B antibody (51020-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using SEC61B Polyclonal antibody (51020-2-AP)

    IF Staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B antibody (51020-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SEC61B Polyclonal antibody (51020-2-AP)

    IF Staining of HepG2 using 51020-2-AP (same clone as 51020-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 51020-2-AP (SEC61B antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

51020-2-PBS targets SEC61B in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Sec61 beta subunit
Calculated molecular weight 10 kDa
Observed molecular weight 10-15 kDa
GenBank accession numberBC001734
Gene Symbol SEC61B
Gene ID (NCBI) 10952
RRIDAB_2186413
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP60468
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).