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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using SEC61 Alpha Polyclonal antibody (24935-1-AP)

    WB analysis of mouse brain using 24935-1-AP (same clone as 24935-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24935-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using SEC61 Alpha Polyclonal antibody (24935-1-AP)

    WB analysis of mouse liver using 24935-1-AP (same clone as 24935-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61alpha antibody at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 24935-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using SEC61 Alpha Polyclonal antibody (24935-1-AP)

    WB analysis of U2OS using 24935-1-AP (same clone as 24935-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61 Alpha antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24935-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SEC61 Alpha Polyclonal antibody (24935-1-AP)

    WB analysis of HEK-293 using 24935-1-AP (same clone as 24935-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24935-1-AP (SEC61alpha antibody at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 24935-1-PBS in a different storage buffer formulation.

SEC61 Alpha Polyclonal antibody, PBS Only

SEC61 Alpha Polyclonal Antibody for WB, Indirect ELISA
Cat No. 24935-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

SEC61A2, Sec61 alpha-2, Protein transport protein Sec61 subunit alpha isoform 2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24935-1-PBS targets SEC61 Alpha in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20779

Product name: Recombinant human SEC61A2 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 190-418 aa of BC026179

Sequence: TIVWKAFSPTTINTGRGTEFEGAVIALFHLLATRTDKVRALREAFYRQNLPNLMNLIATVFVFAVVIYFQGFRVDLPIKSARYRGQYSSYPIKLFYTSNIPIILQSALVSNLYVISQMLSVRFSGNFLVNLLGQWADVSGGGPARSYPVGGLCYYLSPPESMGAIFEDPVHVVVYIIFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRDTSMVHELNSARA

相同性解析による交差性が予測される生物種
Full Name Sec61 alpha 2 subunit (S. cerevisiae)
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC026179
Gene Symbol SEC61 Alpha
Gene ID (NCBI) 55176
RRIDAB_2879807
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H9S3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The SEC61 complex is a major component of the endoplasmic reticulum (ER) translocation site (PMID: 7720858). This complex is composed of three subunits: SEC61alpha, SEC61beta and SEC61gamma. SEC61alpha is a polytopic integral membrane protein. It is a core component of the ER translocation complex, playing a crucial role in the insertion of secretory and membrane polypeptides into the ER (PMID: 9512475).

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