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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using SEC6 Polyclonal antibody (14703-1-AP)

    WB analysis of human brain using 14703-1-AP (same clone as 14703-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:86 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SEC6 Polyclonal antibody (14703-1-AP)

    WB analysis using 14703-1-AP (same clone as 14703-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SEC6 Polyclonal antibody (14703-1-AP)

    WB analysis of HeLa using 14703-1-AP (same clone as 14703-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using SEC6 Polyclonal antibody (14703-1-AP)

    WB analysis of COLO 320 using 14703-1-AP (same clone as 14703-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SEC6 Polyclonal antibody (14703-1-AP)

    WB analysis of HepG2 using 14703-1-AP (same clone as 14703-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14703-1-AP (SEC6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SEC6 Polyclonal antibody (14703-1-AP)

    IHC staining of human gliomas using 14703-1-AP (same clone as 14703-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14703-1-AP (SEC6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SEC6 Polyclonal antibody (14703-1-AP)

    IHC staining of human gliomas using 14703-1-AP (same clone as 14703-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14703-1-AP (SEC6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using SEC6 Polyclonal antibody (14703-1-AP)

    IHC staining of human gliomas using 14703-1-AP (same clone as 14703-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 14703-1-AP (SEC6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SEC6 Polyclonal antibody (14703-1-AP)

    IF Staining of HepG2 using 14703-1-AP (same clone as 14703-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 14703-1-AP (SEC6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14703-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SEC6 Polyclonal antibody, PBS Only

SEC6 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14703-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EXOC3, Exocyst complex component 3, Exocyst complex component Sec6, SEC6L1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14703-1-PBS targets SEC6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6383

Product name: Recombinant human SEC6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 381-745 aa of BC064569

Sequence: DTYMSTLTSNIIAWLRKALETDKKDWVKETEPEADQDGYYQTTLPAIVFQMFEQNLQVAAQISEDLKTKVLVLCLQQMNSFLSRYKDEAQLYKEEHLRNRQHPHCYVQYMIAIINNCQTFKESIVSLKRKYLKNEVEEGVSPSQPSMDGILDAIAKEGCSGLLEEVFLDLEQHLNELMTKKWLLGSNAVDIICVTVEDYFNDFAKIKKPYKKRMTAEAHRRVVVEYLRAVMQKRISFRSPEERKEGAEKMVREAEQLRFLFRKLASGFGEDVDGYCDTIVAVAEVIKLTDPSLLYLEVSTLVSKYPDIRDDHIGALLAVRGDASRDMKQTIMETLEQGPAQASPSYVPLFKDIVVPSLNVAKLLK

相同性解析による交差性が予測される生物種
Full Name exocyst complex component 3
Calculated molecular weight 87 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC064569
Gene Symbol SEC6
Gene ID (NCBI) 11336
RRIDAB_2101542
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60645
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Exoc3 (Sec6) is a component of the exocyst (Sec6/8) complex involved in vesicle trafficking at neural synapses and vesicle targeting in polarized epithelial cells. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. This complex is composed of eight proteins (Sec3, Sec5, Sec6, Sec8, Sec10, and Sec15, Exo70 and Exo84), with molecular weights ranging from 70 to 144 kDa.

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