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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SEC5/EXOC2 Monoclonal antibody (66011-1-Ig)

    WB analysis of HEK-293 using 66011-1-Ig (same clone as 66011-1-PBS)

    WB result of SEC5/EXOC2 antibody (66011-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEC5/EXOC2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using SEC5/EXOC2 Monoclonal antibody (66011-1-Ig)

    WB analysis of mouse brain using 66011-1-Ig (same clone as 66011-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using SEC5/EXOC2 Monoclonal antibody (66011-1-Ig)

    WB analysis of pig brain using 66011-1-Ig (same clone as 66011-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig cerebellum tissue using SEC5/EXOC2 Monoclonal antibody (66011-1-Ig)

    WB analysis of pig cerebellum using 66011-1-Ig (same clone as 66011-1-PBS)

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using SEC5/EXOC2 Monoclonal antibody (66011-1-Ig)

    WB analysis of rat brain using 66011-1-Ig (same clone as 66011-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat cerebellum tissue using SEC5/EXOC2 Monoclonal antibody (66011-1-Ig)

    WB analysis of rat cerebellum using 66011-1-Ig (same clone as 66011-1-PBS)

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using SEC5/EXOC2 Monoclonal antibody (66011-1-Ig)

    WB analysis of human brain using 66011-1-Ig (same clone as 66011-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SEC5/EXOC2 Monoclonal antibody (66011-1-Ig)

    IP experiment of mouse brain using 66011-1-Ig (same clone as 66011-1-PBS)

    IP result of anti-SEC5/EXOC2 (IP:66011-1-Ig, 5ug; Detection:66011-1-Ig 1:500) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SEC5/EXOC2 Monoclonal antibody (66011-1-Ig)

    IHC staining of human breast cancer using 66011-1-Ig (same clone as 66011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SEC5/EXOC2 Monoclonal antibody (66011-1-Ig)

    IHC staining of human breast cancer using 66011-1-Ig (same clone as 66011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SEC5/EXOC2 Monoclonal antibody (66011-1-Ig)

    IHC staining of human liver cancer using 66011-1-Ig (same clone as 66011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SEC5/EXOC2 Monoclonal antibody (66011-1-Ig)

    IHC staining of human liver cancer using 66011-1-Ig (same clone as 66011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MDA-MB-231 cells using SEC5/EXOC2 Monoclonal antibody (66011-1-Ig)

    IF Staining of MDA-MB-231 using 66011-1-Ig (same clone as 66011-1-PBS)

    Immunostaining of exocyst protein Sec5 (66011-1-Ig, red) in MDA-MB-231 cells. The image was credited by Dr. Hae Lin Jang from Harvard Medical School. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SEC5/EXOC2 Monoclonal antibody (66011-1-Ig)

    IF Staining of HeLa using 66011-1-Ig (same clone as 66011-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC5/EXOC2 antibody (66011-1-Ig, Clone: 1C11G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SEC5/EXOC2 Monoclonal antibody, PBS Only

SEC5/EXOC2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66011-1-PBS
Clone No.1C11G2

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EXOC2, exocyst complex component 2, Exocyst complex component Sec5, SEC5, SEC5/EXOC2, SEC5L1, Sec5p

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66011-1-PBS targets SEC5/EXOC2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17866

Product name: Recombinant human SEC5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 456-806 aa of BC016918

Sequence: TIQDLILDLRVRCVMATLQHTAEEIKRLAEKEDWIVDNEGLTSLPCQFEQCIVCSLQSLKGVLECKPGEASVFQQPKTQEEVCQLSINIMQVFIYCLEQLSTKPDADIDTTHLSVDVSSPDLFGSIHEDFSLTSEQRLLIVLSNCCYLERHTFLNIAEHFEKHNFQGIEKITQVSMASLKELDQRLFENYIELKADPIVGSLEPGIYAGYFDWKDCLPPTGVRNYLKEALVNIIAVHAEVFTISKELVPRVLSKVIEAVSEELSRLMQCVSSFSKNGALQARLEICALRDTVAVYLTPESKSSFKQALEALPQLSSGADKKLLEELLNKFKSSMHLQLTCFQAASSTMMKT

相同性解析による交差性が予測される生物種
Full Name exocyst complex component 2
Calculated molecular weight 924 aa, 104 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC016918
Gene Symbol SEC5
Gene ID (NCBI) 55770
RRIDAB_11042317
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ96KP1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

EXOC2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXOC2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXOC2 mRNA is widely expressed with highest levels in brain and placenta.

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