Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of mouse brain using 12751-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human ileum using 12751-1-AP
human ileum tissue were subjected to SDS PAGE followed by western blot with 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 12751-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 12751-1-AP
IP result of anti-SEC5/EXOC2 (IP:12751-1-AP, 5ug; Detection:12751-1-AP 1:500) with mouse brain tissue lysate 3000ug.
IHC staining of human breast cancer using 12751-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:100 (under 10x lens).
IF Staining of HeLa using 12751-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12751-1-AP (SEC5/EXOC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

SEC5/EXOC2 Polyclonal antibody

SEC5/EXOC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12751-1-AP

Publications(21)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

SEC5L1, SEC5, Exocyst complex component Sec5, Exocyst complex component 2, EXOC2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (21)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, human brain tissue, human ileum tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 8 publications below
WB	See 19 publications below
IHC	See 1 publications below
IF	See 7 publications below
IP	See 1 publications below
CoIP	See 2 publications below

Product Information

12751-1-AP targets SEC5/EXOC2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3147 Product name: Recombinant human SEC5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 456-806 aa of BC016918 Sequence: TIQDLILDLRVRCVMATLQHTAEEIKRLAEKEDWIVDNEGLTSLPCQFEQCIVCSLQSLKGVLECKPGEASVFQQPKTQEEVCQLSINIMQVFIYCLEQLSTKPDADIDTTHLSVDVSSPDLFGSIHEDFSLTSEQRLLIVLSNCCYLERHTFLNIAEHFEKHNFQGIEKITQVSMASLKELDQRLFENYIELKADPIVGSLEPGIYAGYFDWKDCLPPTGVRNYLKEALVNIIAVHAEVFTISKELVPRVLSKVIEAVSEELSRLMQCVSSFSKNGALQARLEICALRDTVAVYLTPESKSSFKQALEALPQLSSGADKKLLEELLNKFKSSMHLQLTCFQAASSTMMKT 相同性解析による交差性が予測される生物種
Full Name	exocyst complex component 2
Calculated molecular weight	924 aa, 104 kDa
Observed molecular weight	95-100 kDa
GenBank accession number	BC016918
Gene Symbol	SEC5
Gene ID (NCBI)	55770
RRID	AB_2262528
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96KP1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

EXOC2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXOC2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXOC2 mRNA is widely expressed with highest levels in brain and placenta.

Protocols

Product Specific Protocols
IF protocol for SEC5/EXOC2 antibody 12751-1-AP	Download protocol
IHC protocol for SEC5/EXOC2 antibody 12751-1-AP	Download protocol
IP protocol for SEC5/EXOC2 antibody 12751-1-AP	Download protocol
WB protocol for SEC5/EXOC2 antibody 12751-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell A kinase-independent role for EGF receptor in autophagy initiation. Authors - Xiaojun Tan KD Validated View Article
human	WB	Nat Cell Biol M-Sec promotes membrane nanotube formation by interacting with Ral and the exocyst complex. Authors - Hase Koji K KD Validated View Article
human	WB,IF	Nat Cell Biol The deubiquitylase USP33 discriminates between RALB functions in autophagy and innate immune response. Authors - Simicek Michal M KD Validated View Article
human	WB	Blood vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2. Authors - Anish Vaibhav Sharda View Article
mouse	WB,IP	Mol Cell The anti-inflammatory TIPE2 is an inhibitor of the oncogenic Ras. Authors - Gus-Brautbar Yael Y View Article
mouse	WB,CoIP	Sci Signal Phosphorylation of the exocyst protein Exo84 by TBK1 promotes insulin-stimulated GLUT4 trafficking. Authors - Maeran Uhm View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse brain using 12751-1-AP

WB analysis of human ileum using 12751-1-AP

WB analysis of human brain using 12751-1-AP

IP experiment of mouse brain using 12751-1-AP

IHC staining of human breast cancer using 12751-1-AP

IF Staining of HeLa using 12751-1-AP

SEC5/EXOC2 Polyclonal antibody

SEC5/EXOC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A kinase-independent role for EGF receptor in autophagy initiation.

M-Sec promotes membrane nanotube formation by interacting with Ral and the exocyst complex.

The deubiquitylase USP33 discriminates between RALB functions in autophagy and innate immune response.

vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2.

The anti-inflammatory TIPE2 is an inhibitor of the oncogenic Ras.

Phosphorylation of the exocyst protein Exo84 by TBK1 promotes insulin-stimulated GLUT4 trafficking.