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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEC31A Recombinant antibody (84942-4-RR)

    WB analysis using 84942-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84942-4-RR (SEC31A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using SEC31A Recombinant antibody (84942-4-RR)

    IP experiment of HeLa using 84942-4-RR

    IP result of anti-SEC31A (IP:84942-4-RR, 4ug; Detection:84942-4-RR 1:4000) with HeLa cells lysate 1880 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SEC31A Recombinant antibody (84942-4-RR)

    IF Staining of HeLa using 84942-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SEC31A antibody (84942-4-RR, Clone: 242531E2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84942-4-RR

    Affinity and Kinetic Characterization of 84942-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84942-4-RR against Human SEC31A were performed. The affinity constant is 12.4 nM.

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SEC31A Recombinant antibody

SEC31A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, ELISA
Cat No. 84942-4-RR
Clone No.242531E2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Synonyms

242531E2, ABP125, ABP130, KIAA0905, SEC31 like protein 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84942-4-RR targets SEC31A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SEC31A fusion protein Ag12314 相同性解析による交差性が予測される生物種
Full Name SEC31 homolog A (S. cerevisiae)
Calculated molecular weight 1220 aa, 133 kDa
Observed molecular weight 118-140 kDa
GenBank accession numberBC084583
Gene Symbol SEC31A
Gene ID (NCBI) 22872
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO94979
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). The sec31 subunit of the coat complex has been shown to be essential for vesicle formation and ER-Golgi transport (PMID: 10788476). Two mammalian homologs of yeast sec31p, Sec31A and Sec31B, share about 40% amino acid identity. Sec31A transcripts are ubiquitously and abundantly expressed.

Protocols

Product Specific Protocols
WB protocol for SEC31A antibody 84942-4-RRDownload protocol
IF protocol for SEC31A antibody 84942-4-RRDownload protocol
IP protocol for SEC31A antibody 84942-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols