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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEC23IP Polyclonal antibody (20892-1-AP)

    WB analysis using 20892-1-AP (same clone as 20892-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20892-1-AP (SEC23IP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20892-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using SEC23IP Polyclonal antibody (20892-1-AP)

    WB analysis of mouse kidney using 20892-1-AP (same clone as 20892-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20892-1-AP (SEC23IP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20892-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using SEC23IP Polyclonal antibody (20892-1-AP)

    IP experiment of HeLa using 20892-1-AP (same clone as 20892-1-PBS)

    IP result of anti-SEC23IP (IP:20892-1-AP, 4ug; Detection:20892-1-AP 1:1000) with HeLa cells lysate 3600ug. This data was developed using the same antibody clone with 20892-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SEC23IP Polyclonal antibody (20892-1-AP)

    IHC staining of mouse brain using 20892-1-AP (same clone as 20892-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20892-1-AP (SEC23IP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20892-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SEC23IP Polyclonal antibody (20892-1-AP)

    IHC staining of mouse brain using 20892-1-AP (same clone as 20892-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20892-1-AP (SEC23IP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20892-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SEC23IP Polyclonal antibody (20892-1-AP)

    IF Staining of HeLa using 20892-1-AP (same clone as 20892-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC23IP antibody (20892-1-AP) at dilution of 1:0 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20892-1-PBS in a different storage buffer formulation.

SEC23IP Polyclonal antibody, PBS Only

SEC23IP Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 20892-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

MSTP053, p125, SEC23-interacting protein

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20892-1-PBS targets SEC23IP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14916

Product name: Recombinant human SEC23IP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 651-1000 aa of BC063800

Sequence: ETLEALSLSEYFSTFEKEKIDMESLLMCTVDDLKEMGIPLGPRKKIANFVEHKAAKLKKAASEKKAVAATSTKGQEQSAQKTKDMASLPSESNEPKRKLPVGACVSSVCVNYESFEVGAGQVSVAYNSLDFEPEIFFALGSPIAMFLTIRGVDRIDENYSLPTCKGFFNIYHPLDPVAYRLEPMIVPDLDLKAVLIPHHKGRKRLHLELKESLSRMGSDLKQGFISSLKSAWQTLNEFARAHTSSTQLQEELEKVANQIKEEEEKQVVEAEKVVESPDFSKDEDYLGKVGMLNGGRRIDYVLQEKPIESFNEYLFALQSHLCYWESEDTALLLLKEIYRTMNISPEQPQH

相同性解析による交差性が予測される生物種
Full Name SEC23 interacting protein
Calculated molecular weight 1000 aa, 111 kDa
Observed molecular weight 125 kDa
GenBank accession numberBC063800
Gene Symbol SEC23IP
Gene ID (NCBI) 11196
RRIDAB_10896458
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6Y8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SEC23-interacting protein (SEC23IP), also known as p125, is a member of the phosphatidic acid preferring-phospholipase A1 family. SEC23IP interacts with Sec23, a coat component of COPII vesicles which are involved in protein export from the endoplasmic reticulum (ER). SEC23IP localizes to ER exit sites and participates in the organization of this compartment (PMID: 15623529). It may have a role in the early stage of the secretory pathway (PMID: 10400679).

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