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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEC14L3 Polyclonal antibody (32098-1-AP)

    WB analysis using 32098-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32098-1-AP (SEC14L3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SEC14L3 Polyclonal antibody (32098-1-AP)

    IF Staining of HeLa using 32098-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC14L3 antibody (32098-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

SEC14L3 Polyclonal antibody

SEC14L3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32098-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Tocopherol-associated protein 2, TAP2, SEC14-like protein 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, mouse lung tissue, rat lung tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32098-1-AP targets SEC14L3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC14L3 fusion protein Ag36792 相同性解析による交差性が予測される生物種
Full Name SEC14-like 3 (S. cerevisiae)
Calculated molecular weight 46 kDa
Observed molecular weight45 kDa
GenBank accession numberBC069641
Gene Symbol SEC14L3
Gene ID (NCBI) 266629
RRIDAB_3670189
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9UDX4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SEC14L3 is highly similar to the protein encoded by the Saccharomyces cerevisiae SEC14 gene. The SEC14 protein is a phophatidylinositol transfer protein that is essential for biogenesis of Golgi-derived transport vesicles, and thus is required for the export of yeast secretory proteins from the Golgi complex. The specific function of this protein has not yet been determined. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Protocols

Product Specific Protocols
WB protocol for SEC14L3 antibody 32098-1-APDownload protocol
IF protocol for SEC14L3 antibody 32098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols