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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEC14L2 Polyclonal antibody (32593-1-AP)

    WB analysis using 32593-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32593-1-AP (SEC14L2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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SEC14L2 Polyclonal antibody

SEC14L2 Polyclonal Antibody for WB, ELISA
Cat No. 32593-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Alpha-tocopherol-associated protein, C22orf6, hTAP, KIAA1186, KIAA1658

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, RKO cells, fetal human brain tissue, mouse brain tissue, mouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32593-1-AP targets SEC14L2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC14L2 fusion protein Ag35592 相同性解析による交差性が予測される生物種
Full Name SEC14-like 2 (S. cerevisiae)
Calculated molecular weight 46 kDa
Observed molecular weight44 kDa
GenBank accession numberBC058915
Gene Symbol SEC14L2
Gene ID (NCBI) 23541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO76054
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SEC14L2 (SEC14-like lipid-binding protein 2) is a cytoplasmic protein that belongs to the lipid-binding protein family. It plays an important role in a variety of cellular processes, including endosome division and lipid metabolism. SEC14L2 is able to bind phosphatidylinositol (PtdIns) and regulate its intracellular distribution, which is essential for normal endosome division. It has been shown that SEC14L2 regulates the endosome division process by promoting the conversion of PtdIns4P to PtdIns3P. In addition, SEC14L2 plays a role in the cholesterol biosynthesis pathway by stimulating squalene monooxygenase activity. In terms of viral infection, SEC14L2 is essential for hepatitis C virus (HCV) replication, and its expression promotes HCV replication in cells.

Protocols

Product Specific Protocols
WB protocol for SEC14L2 antibody 32593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols