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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEC11C Polyclonal antibody (16901-1-AP)

    WB analysis using 16901-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16901-1-AP (SEC11C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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SEC11C Polyclonal antibody

SEC11C Polyclonal Antibody for WB, ELISA
Cat No. 16901-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

EC:3.4.21.89, SEC11 homolog C, SEC11 like protein 3, SEC11L3, SEC11-like protein 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, mouse kidney tissue, rat kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16901-1-AP targets SEC11C in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC11C fusion protein Ag10442 相同性解析による交差性が予測される生物種
Full Name SEC11 homolog C (S. cerevisiae)
Calculated molecular weight 192 aa, 22 kDa
Observed molecular weight16-22 kDa
GenBank accession numberBC009703
Gene Symbol SEC11C
Gene ID (NCBI) 90701
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BY50
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SEC11C (Signal peptidase complex catalytic subunit SEC11C), also known as SPC21. It is located in the Endoplasmic reticulum membrane. Catalytic component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum 1. Specifically cleaves N-terminal signal peptides that contain a hydrophobic alpha-helix (h-region) shorter than 18-20 amino acids. The molecular weight of SEC11C is 22 kDa. It has been reported that its molecular weight is 16kDa (PMID: 38183983).

Protocols

Product Specific Protocols
WB protocol for SEC11C antibody 16901-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols