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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SDS Polyclonal antibody

SDS Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16594-1-AP

Synonyms

L serine deaminase, L threonine dehydratase, SDH, SDS, serine dehydratase, TDH

Validation Data Gallery

Applications:
  • WB analysis using 16594-1-AP

    WB analysis using 16594-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16594-1-AP (SDS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 16594-1-AP

    WB analysis using 16594-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16594-1-AP (SDS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16594-1-AP

    IHC staining of human liver using 16594-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16594-1-AP (SDS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 16594-1-AP

    IHC staining of human liver using 16594-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16594-1-AP (SDS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16594-1-AP targets SDS in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SDS fusion protein Ag9704 相同性解析による交差性が予測される生物種
Full Name serine dehydratase
Calculated molecular weight 218 aa, 23 kDa
Observed molecular weight35 kDa; 70 kDa
GenBank accession numberBC020750
Gene symbol SDS
Gene ID (NCBI) 10993
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

L-Serine dehydratase (SDS ; SDH) catalyzes the pyridoxal phosphate (PLP) dependent deamination of l-serine to yield pyruvate. In mammals, SDH is found predominantly in the liver. Extensive studies have been carried out on SDH from rat and the enzyme has been found to play an important role in gluconeogenesis; its activity is induced by the consumption of high-protein diets, starvation and other treatments. The enzyme exists as a dimer of identical subunits, with each subunit exhibiting a bilobal architecture. (PMID: 25380533, PMID: 21878319, PMID: 14646100)

Protocols

Product Specific Protocols
WB protocol for SDS antibody 16594-1-APDownload protocol
IHC protocol for SDS antibody 16594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols