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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SDH5 Polyclonal antibody (19906-1-AP)

    WB analysis using 19906-1-AP (same clone as 19906-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19906-1-AP (SDH5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19906-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using SDH5 Polyclonal antibody (19906-1-AP)

    IHC staining of human testis using 19906-1-AP (same clone as 19906-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19906-1-AP (SDH5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19906-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using SDH5 Polyclonal antibody (19906-1-AP)

    IHC staining of human testis using 19906-1-AP (same clone as 19906-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19906-1-AP (SDH5 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19906-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using SDH5 Polyclonal antibody (19906-1-AP)

    IHC staining of human skin cancer using 19906-1-AP (same clone as 19906-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19906-1-AP (SDH5 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19906-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using SDH5 Polyclonal antibody (19906-1-AP)

    IHC staining of human skin cancer using 19906-1-AP (same clone as 19906-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19906-1-AP (SDH5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19906-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SDH5 Polyclonal antibody (19906-1-AP)

    IF Staining of HeLa using 19906-1-AP (same clone as 19906-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SDH5 antibody (19906-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19906-1-PBS in a different storage buffer formulation.

SDH5 Polyclonal antibody, PBS Only

SDH5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 19906-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19906-1-PBS targets SDH5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13893

Product name: Recombinant human C11orf79 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-166 aa of BC002331

Sequence: MAVSTVFSTSSLMLALSRHSLLSPLLSVTSFRRFYRGDSPTDSQKDMIEIPLPPWQERTDESIETKRARLLYESRKRGMLENCILLSLFAKEHLQHMTEKQLNLYDRLINEPSNDWDIYYWATEAKPAPEIFENEVMALLRDFAKNKNKEQRLRAPDLEYLFEKPR

相同性解析による交差性が予測される生物種
Full Name chromosome 11 open reading frame 79
Calculated molecular weight 166 aa, 20 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC002331
Gene Symbol SDH5
Gene ID (NCBI) 54949
RRIDAB_2878621
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NX18
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SDH5, also named asC11orf79, SDHAF2 and SDHAF2, is a mitochondrial protein needed for the flavination of a succinate dehydrogenase complex subunit required for activity of the complex. SDH5 has been shown to be mutated in patients with paragangliomas, also known as familial non-chromaffin paragangliomas type 2. Catalog#19906-1-AP is a rabbit polyclonal antibody raised against the full-length of human SDH5.

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