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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using SDF4 Polyclonal antibody (10517-1-AP)

    IHC staining of mouse testis using 10517-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10517-1-AP (SDF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SDF4 Polyclonal antibody (10517-1-AP)

    IHC staining of mouse testis using 10517-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10517-1-AP (SDF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using SDF4 Polyclonal antibody (10517-1-AP)

    IHC staining of human colon using 10517-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10517-1-AP (SDF4 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using SDF4 Polyclonal antibody (10517-1-AP)

    IHC staining of human colon using 10517-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10517-1-AP (SDF4 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using SDF4 Polyclonal antibody (10517-1-AP)

    IHC staining of human pancreas using 10517-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10517-1-AP (SDF4 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using SDF4 Polyclonal antibody (10517-1-AP)

    IHC staining of human pancreas using 10517-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10517-1-AP (SDF4 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using SDF4 Polyclonal antibody (10517-1-AP)

    IF Staining of mouse testis using 10517-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SDF4 antibody (10517-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using SDF4 Polyclonal antibody (10517-1-AP)

    IF Staining of mouse testis using 10517-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SDF4 antibody (10517-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SDF4 Polyclonal antibody

SDF4 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 10517-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, CoIP, ELISA

Synonyms

45 kDa calcium binding protein, 45 kDa calcium-binding protein, Cab45, PSEC0034, SDF 4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse testis tissue, human pancreas tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10517-1-AP targets SDF4 in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0806

Product name: Recombinant human SDF4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 155-362 aa of BC006211

Sequence: GHVSWDEYKVKFLASKGHSEKEVADAIRLNEELKVDEETQEVLENLKDRWYQADSPPADLLLTEEEFLSFLHPEHSRGMLRFMVKEIVRDLDQDGDKQLSVPEFISLPVGTVENQQGQDIDDNWVKDRKKEFEELIDSNHDGIVTAEELESYMDPMNEYNALNEAKQMIAVADENQNHHLEPEEVLKYSEFFTGSKLVDYARSVHEEF

相同性解析による交差性が予測される生物種
Full Name stromal cell derived factor 4
Calculated molecular weight 42 kDa
GenBank accession numberBC006211
Gene Symbol SDF4
Gene ID (NCBI) 51150
RRIDAB_2301345
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRK5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for SDF4 antibody 10517-1-APDownload protocol
IHC protocol for SDF4 antibody 10517-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF,CoIP

Cell Death Discov

Fibroblast CEBPD/SDF4 axis in response to chemotherapy-induced angiogenesis through CXCR4.

Authors - Jhih-Ying Chi
View Article
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