Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, THP-1 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66284-1-Ig targets SDCCAG8 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4264 Product name: Recombinant human SDCCAG8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-360 aa of BC032454 Sequence: MAKSPENSTLEEILGQYQRSLREHASRSIHQLTCALKEGDVTIGEDAPNLSFSTSVGNEDARTAWPELQQSHAVNQLKDLLRQQADKESEVSPSRRRKMSPLRSLEHEETNMPTMHDLVHTINDQSQYIHHLEAEVKFCKEELSGMKNKIQVVVLENEGLQQQLKSQRQEETLREQTLLDASGNMHNSWITTGEDSGVGETSKRPFSHDNADFGKAASAGEQLELEKLKLTYEEKCEIEESQLKFLRNDLAEYQRTCEDLKEQLKHKEFLLAANTCNRVGGLCLKCAQHEAVLSQTHTNVHMQTIERLVKERDDLMSALVSVRSSLADTQQREASAYEQVKQVLQISEEANFEKTKHPSQ 相同性解析による交差性が予測される生物種 |
| Full Name | serologically defined colon cancer antigen 8 |
| Calculated molecular weight | 713 aa, 83 kDa |
| Observed molecular weight | 83 kDa |
| GenBank accession number | BC032454 |
| Gene Symbol | SDCCAG8 |
| Gene ID (NCBI) | 10806 |
| RRID | AB_2881667 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q86SQ7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SDCCAG8, also named as CCCAP and NY-CO-8, plays a role in the establishment of cell polarity and epithelial lumen formation. It may play a role in ciliogenesis. Loss of SDCCAG8 function as a cause of a retinal-renal ciliopathy and validates exome capture analysis for broadly heterogeneous single-gene disorders. SDCCAG8 is localized at both centrioles and interacts directly with OFD1 (oral-facial-digital syndrome 1), which is associated with NPHP-RC.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SDCCAG8 antibody 66284-1-Ig | Download protocol |
| WB protocol for SDCCAG8 antibody 66284-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
