Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse testis tissue, rat testis tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 8 publications below |
| IHC | See 3 publications below |
| IF | See 8 publications below |
Product Information
15969-1-AP targets SDCCAG3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8745 Product name: Recombinant human SDCCAG3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 118-412 aa of BC014515 Sequence: LGLDHNSPPSQTGGYGLEYQQPFFEDPTGAGDLLDGEEDEDTGWSGAYLPSAIEQTHPERVPAGTSPCSTYLSFFSTPSELAGPESLPSWALSDTDSRVSPASPAGSPSADFAVHGESLGDRHLRTLQISYDALKDENSKLRRKLNEVQSFSEAQTEMVRTLERKLEAKMIKEESDYHDLESVVQQVEQNLELMTKRAVKAENHVVKLKQEISLLQAQVSNFQRENEALRCGQGASLTVVKQNADVALQNLRVVMNSAQASIKQLVSGAETLNLVAEILKSIDRISEIKDEEEDS 相同性解析による交差性が予測される生物種 |
| Full Name | serologically defined colon cancer antigen 3 |
| Calculated molecular weight | 412 aa, 46 kDa |
| Observed molecular weight | 48-60 kDa |
| GenBank accession number | BC014515 |
| Gene Symbol | SDCCAG3 |
| Gene ID (NCBI) | 10807 |
| RRID | AB_2183415 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96C92 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SDCCAG3, also named as NY-CO-3, is identified as serologically defined colon cancer antigen-3. It is an endosomal protein which is involved in the regulation of cytokinesis. SDCCAG3 is involved in modulation of TNF response. This gene has four isoforms with MW 40-50 kDa. Observed MW this protein is 48-60 kDa may due to phosphorylation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SDCCAG3 antibody 15969-1-AP | Download protocol |
| IHC protocol for SDCCAG3 antibody 15969-1-AP | Download protocol |
| IF protocol for SDCCAG3 antibody 15969-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Apoptotic signalling targets the post-endocytic sorting machinery of the death receptor Fas/CD95. | ||
Curr Biol Retromer Binding to FAM21 and the WASH Complex Is Perturbed by the Parkinson Disease-Linked VPS35(D620N) Mutation. | ||
Oncogene The serologically defined colon cancer antigen-3 interacts with the protein tyrosine phosphatase PTPN13 and is involved in the regulation of cytokinesis.
| ||
J Cell Sci Identification of molecular heterogeneity in SNX27-retromer-mediated endosome-to-plasma membrane recycling.
| ||
Sci Rep The serologically defined colon cancer antigen-3 (SDCCAG3) is involved in the regulation of ciliogenesis.
| ||