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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SDCCAG1 Polyclonal antibody (11840-1-AP)

    WB analysis using 11840-1-AP (same clone as 11840-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11840-1-AP (SDCCAG1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11840-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A549 cells using SDCCAG1 Polyclonal antibody (11840-1-AP)

    IP experiment of A549 using 11840-1-AP (same clone as 11840-1-PBS)

    IP result of anti-SDCCAG1 (IP:11840-1-AP, 4ug; Detection:11840-1-AP 1:500) with A549 cells lysate 1800ug. This data was developed using the same antibody clone with 11840-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SDCCAG1 Polyclonal antibody (11840-1-AP)

    IHC staining of human lung cancer using 11840-1-AP (same clone as 11840-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11840-1-AP (SDCCAG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11840-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SDCCAG1 Polyclonal antibody (11840-1-AP)

    IHC staining of human lung cancer using 11840-1-AP (same clone as 11840-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11840-1-AP (SDCCAG1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11840-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SDCCAG1 Polyclonal antibody, PBS Only

SDCCAG1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 11840-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11840-1-PBS targets SDCCAG1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2467

Product name: Recombinant human SDCCAG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 48-397 aa of BC020794

Sequence: EDITLQSGRDELNEELIQEESSEDEGEYEEVRKDQDSVGEMKDEGEETLNYPDTTIDLSHLQPQRSIQKLASKEESSNSSDSKSQSRRHLSAKERREMKKKKLPSDSGDLEALEGKDKEKESTVHIETHQNTSKNVAAVQPMKRGQKSKMKKMKEKYKDQDEEDRELIMKLLGSAGSNKEEKGKKGKKGKTKDEPVKKQPQKPRGGQRVSDNIKKETPFLEVITHELQDFAVDDPHDDKEEQDLDQQGNEENLFDSLTGQPHPEDVLLFAIPICAPYTTMTNYKYKVKLTPGVQKKGKAAKTALNSFMHSKEATAREKDLFRSVKDTDLSRNIPGKVKVSAPNLLNVKRK

相同性解析による交差性が予測される生物種
Full Name serologically defined colon cancer antigen 1
Calculated molecular weight 1076 aa, 123 kDa
Observed molecular weight 130-140 kDa
GenBank accession numberBC020794
Gene Symbol SDCCAG1
Gene ID (NCBI) 9147
RRIDAB_2183413
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60524
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SDCCAG1, also named as NEMF, is a nuclear export mediator. It can be bound to translocon-engaged 60S subunits on native ER membranes. Human SDCCAG1 has been implicated in lung cancers and it may function as a tumor suppressor gene. SDCCAG1 inactivation contributes to the transformed state of human lung cancer cells (PMID:16103875, 10575219). NEMF is also regarded as a potential biomarker for the detection of active and latent TB infections (PMID: 26818387).

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