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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SDC4 Polyclonal antibody (11820-1-AP)

    WB analysis using 11820-1-AP (same clone as 11820-1-PBS)

    NIH/3T3 lysates were subjected to SDS PAGE followed by western blot with 11820-1-AP (SDC4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11820-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SDC4 Polyclonal antibody (11820-1-AP)

    IHC staining of mouse skeletal muscle using 11820-1-AP (same clone as 11820-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11820-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SDC4 Polyclonal antibody (11820-1-AP)

    IHC staining of mouse skeletal muscle using 11820-1-AP (same clone as 11820-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11820-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SDC4 Polyclonal antibody (11820-1-AP)

    IHC staining of human liver cancer using 11820-1-AP (same clone as 11820-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11820-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SDC4 Polyclonal antibody (11820-1-AP)

    IHC staining of human liver cancer using 11820-1-AP (same clone as 11820-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11820-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SDC4 Polyclonal antibody (11820-1-AP)

    IHC staining of human liver cancer using 11820-1-AP (same clone as 11820-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11820-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SDC4 Polyclonal antibody (11820-1-AP)

    IHC staining of human liver cancer using 11820-1-AP (same clone as 11820-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11820-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SDC4 Polyclonal antibody, PBS Only

SDC4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11820-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Amphiglycan, Ryudocan core protein, SYND4, syndecan 4, Syndecan-4

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11820-1-PBS targets SDC4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2388

Product name: Recombinant human SDC4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-198 aa of BC030805

Sequence: MAPARLFALLLFFVGGVAESIRETEVIDPQDLLEGRYFSGALPDDEDVVGPGQESDDFELSGSGDLDDLEDSMIGPEVVHPLVPLDNHIPERAGSGSQVPTEPKKLEENEVIPKRISPVEESEDVSNKVSMSSTVQGSNIFERTEVLAALIVGGIVGILFAVFLILLLMYRMKKKDEGSYDLGKKPIYKKAPTNEFYA

相同性解析による交差性が予測される生物種
Full Name syndecan 4
Calculated molecular weight 22 kDa
Observed molecular weight 22-26 kDa
GenBank accession numberBC030805
Gene Symbol SDC4
Gene ID (NCBI) 6385
RRIDAB_2877797
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP31431
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SDC4, also known as syndecan 4, is a cell surface proteoglycan that bears heparan sulfate, which is involved in normal wound healing. It has been reported that SDC4 may be associated with keratinocyte proliferation and differentiation in the epidermis. SDC4 also plays a key role in angiogenesis and inflammation. The predicted MW of SDC4 is 22 kDa, and 11820-1-AP antibody recognizes a protein around 24-26 kDa and 48-52 kDa (homodimer), which is similar to the papers.(PMID: 20639201, 21949132; 18638267; 30165731 )

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