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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCYL2 Polyclonal antibody (12325-1-AP)

    WB analysis using 12325-1-AP (same clone as 12325-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using SCYL2 Polyclonal antibody (12325-1-AP)

    WB analysis of NIH/3T3 using 12325-1-AP (same clone as 12325-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse uterus tissue using SCYL2 Polyclonal antibody (12325-1-AP)

    WB analysis of mouse uterus using 12325-1-AP (same clone as 12325-1-PBS)

    mouse uterus tissue were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293T cells using SCYL2 Polyclonal antibody (12325-1-AP)

    WB analysis of HEK-293T using 12325-1-AP (same clone as 12325-1-PBS)

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SCYL2 Polyclonal antibody (12325-1-AP)

    WB analysis of HepG2 using 12325-1-AP (same clone as 12325-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SCYL2 Polyclonal antibody (12325-1-AP)

    WB analysis of HeLa using 12325-1-AP (same clone as 12325-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using SCYL2 Polyclonal antibody (12325-1-AP)

    WB analysis of Jurkat using 12325-1-AP (same clone as 12325-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SCYL2 Polyclonal antibody (12325-1-AP)

    IHC staining of mouse brain using 12325-1-AP (same clone as 12325-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SCYL2 Polyclonal antibody (12325-1-AP)

    IHC staining of mouse brain using 12325-1-AP (same clone as 12325-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using SCYL2 Polyclonal antibody (12325-1-AP)

    IHC staining of mouse cerebellum using 12325-1-AP (same clone as 12325-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using SCYL2 Polyclonal antibody (12325-1-AP)

    IHC staining of mouse cerebellum using 12325-1-AP (same clone as 12325-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

SCYL2 Polyclonal antibody, PBS Only

SCYL2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12325-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12325-1-PBS targets SCYL2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2988

Product name: Recombinant human SCYL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 157-505 aa of BC012387

Sequence: NNLNLNQFNSFISVIKEMLNRLESEHKTKLEQLHIMQEQQKSLDIGNQMNVSEEMKVINIGNQQIDKVFNNIGADLLTGSESENKEDGLQNKHKRASLTLEEKQKLAKEQEQAQKLKSQQPLKPQVHTPVATVKQTKDLTDTLMDNMSSLTSLSVSTPKSSASSTFTSVPSMGIGMMFSTPTDNTKRNLTNGLNANMGFQTSGFNMPVNTNQNFYSSPSTVGVTKMTLGTPPTLPNFNALSVPPAGAKQTQQRPTDMSALNNLFGPQKPKVSMNQLSQQKPNQWLNQFVPPQGSPTMGSSVMGTQMNVIGQSAFGMQGNPFFNPQNFAQPPTTMTNSSSASNDLKDLFG

相同性解析による交差性が予測される生物種
Full Name SCY1-like 2 (S. cerevisiae)
Calculated molecular weight 929 aa, 104 kDa
Observed molecular weight 104 kDa
GenBank accession numberBC012387
Gene Symbol SCYL2
Gene ID (NCBI) 55681
RRIDAB_2071019
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P3W7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.