Validation Data Gallery
Tested Applications
| Positive WB detected in | TT cells, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31577-1-AP targets SCRT1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36215 Product name: Recombinant human SCRT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-195 aa of BC168377 Sequence: MPRSFLVKKVKLDAFSSADLESAYGRARSDLGAPLHDKGYLSDYVGPSSVYDGDAEAALLKGPSPEPMYAAAVRGELGPAAAGSAPPPTPRPELATAAGGYINGDAAVSEGYAADAFFITDGRSRRKASNAGAAAAPSTASAAAPDGDAGGGGGAGGRSLGSGPGGRGGTRAGAGTEARAGPGAAGAGGRHACGE 相同性解析による交差性が予測される生物種 |
| Full Name | scratch homolog 1, zinc finger protein (Drosophila) |
| Observed molecular weight | 36 kDa |
| GenBank accession number | BC168377 |
| Gene Symbol | SCRT1 |
| Gene ID (NCBI) | 83482 |
| RRID | AB_3670042 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9BWW7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SCRT1 (Transcriptional repressor scratch 1), which is expressed in many tissues, especially in brain and adrenal gland. In addition, SCRT1 is also expressed in islet β cells, and its expression is related to insulin secretion ability. SCRT1 is a C2H2 zinc finger transcription inhibitor, which can bind to E-box motif. The encoded protein may promote nerve differentiation and may be related to cancer with neuroendocrine characteristics. The functions of SCRT1 in islet β cells include regulating the expression and secretion of insulin and influencing the proliferation of β cells. SCRT1 may control calcineurin /NFAT signaling pathway by affecting the expression of Syt4, Notch1, Nfatc1 and Nfatc2 genes, which is an important regulator of the growth and function of β cells (PMID: 33309639). The molecular weight of SCRT1 is 36 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SCRT1 antibody 31577-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |