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Validation Data Gallery

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  • Western Blot (WB) analysis of U-251 cells using SCPEP1 Recombinant antibody (83786-4-RR)

    WB analysis of U-251 using 83786-4-RR

    U-251 cells were subjected to SDS PAGE followed by western blot with 83786-4-RR (SCPEP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using SCPEP1 Recombinant antibody (83786-4-RR)

    WB analysis of A431 using 83786-4-RR

    A431 cells were subjected to SDS PAGE followed by western blot with 83786-4-RR (SCPEP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SCPEP1 Recombinant antibody (83786-4-RR)

    IHC staining of human stomach cancer using 83786-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83786-4-RR (SCPEP1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Affinity and Kinetic Characterization of 83786-4-RR

    Affinity and Kinetic Characterization of 83786-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83786-4-RR against Human SCPEP1 were performed. The affinity constant is below 1 pM.

SCPEP1 Recombinant monoclonal antibody

SCPEP1 Recombinant Antibody for WB, IHC, ELISA
Cat No. 83786-4-RR
Clone No.240886B6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

RISC, Retinoid-inducible serine carboxypeptidase, MSTP034, HSCP1, EC:3.4.16.-

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU-251 cells, A431 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83786-4-RR targets SCPEP1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag31381

Product name: Recombinant human SCPEP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 221-452 aa of BC072405

Sequence: LLEDKGLAEVSKVAEQVLNAVNKGLYREATELWGKAEMIIEQNTDGVNFYNILTKSTPTSTMESSLEFTQSHLVCLCQRHVRHLQRDALSQLMNGPIRKKLKIIPEDQSWGGQATNVFVNMEEDFMKPVISIVDELLEAGINVTVYNGQLDLIVDTMGQEAWVRKLKWPELPKFSQLKWKALYSDPKSLETSAFVKSYKNLAFYWILKAGHMVPSDQGDMALKMMRLVTQQE

相同性解析による交差性が予測される生物種
Full Name serine carboxypeptidase 1
Calculated molecular weight 452 aa, 51 kDa
Observed molecular weight33 kDa, 45~51 kDa
GenBank accession numberBC072405
Gene Symbol SCPEP1
Gene ID (NCBI) 59342
RRIDAB_3671378
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9HB40
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SCPEP1 encodes retinoid-inducible serine carboxypeptidase (PMID:12975309). SCPEP1 and Cathepsin A play roles in regulation of vasoconstriction via inactivation of endothelin-1 (PubMed:24586188). The protein is widely expressed during mouse embryogenesis by immunohistochemical (PubMed:16461364). Scpep1 is proteolytically cleaved to a mature enzyme which is smaller than 51 kDa (PMID:16461364).

Protocols

Product Specific Protocols
IHC protocol for SCPEP1 antibody 83786-4-RRDownload protocol
WB protocol for SCPEP1 antibody 83786-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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