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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCPEP1 Polyclonal antibody (29781-1-AP)

    WB analysis using 29781-1-AP (same clone as 29781-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 29781-1-AP (SCPEP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29781-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using SCPEP1 Polyclonal antibody (29781-1-AP)

    IHC staining of mouse embryo using 29781-1-AP (same clone as 29781-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29781-1-AP (SCPEP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29781-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using SCPEP1 Polyclonal antibody (29781-1-AP)

    IHC staining of mouse embryo using 29781-1-AP (same clone as 29781-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29781-1-AP (SCPEP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29781-1-PBS in a different storage buffer formulation.

SCPEP1 Polyclonal antibody, PBS Only

SCPEP1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29781-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

RISC, EC:3.4.16.-, HSCP1, MSTP034, Retinoid-inducible serine carboxypeptidase

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29781-1-PBS targets SCPEP1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31396

Product name: Recombinant human SCPEP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 221-452 aa of BC072405

Sequence: LLEDKGLAEVSKVAEQVLNAVNKGLYREATELWGKAEMIIEQNTDGVNFYNILTKSTPTSTMESSLEFTQSHLVCLCQRHVRHLQRDALSQLMNGPIRKKLKIIPEDQSWGGQATNVFVNMEEDFMKPVISIVDELLEAGINVTVYNGQLDLIVDTMGQEAWVRKLKWPELPKFSQLKWKALYSDPKSLETSAFVKSYKNLAFYWILKAGHMVPSDQGDMALKMMRLVTQQE

相同性解析による交差性が予測される生物種
Full Name serine carboxypeptidase 1
Calculated molecular weight 452 aa, 51 kDa
Observed molecular weight30 kDa, 51 kDa
GenBank accession numberBC072405
Gene Symbol SCPEP1
Gene ID (NCBI) 59342
RRIDAB_2923608
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HB40
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SCPEP1 is synthesized as a ~51-55 kDa precursor (zymogen) containing a signal peptide and five N-glycosylation sites. During lysosomal maturation, it undergoes proteolytic cleavage and N-glycan trimming, producing multiple forms that can appear at ~30 kDa on WB. (PMID: 19187242, 16461364)

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