Validation Data Gallery
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Tested Applications
| Positive WB detected in | U-87 MG cells, HeLa cells, Ramos cells, C6 cells, mouse brain tissue, human heart tissue |
| Positive IHC detected in | rat dorsal root ganglion tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83027-4-RR targets SCN4B in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | SCN4B fusion protein Ag34123 相同性解析による交差性が予測される生物種 |
| Full Name | sodium channel, voltage-gated, type IV, beta |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 37 kDa |
| GenBank accession number | NM_174934.3 |
| Gene Symbol | SCN4B |
| Gene ID (NCBI) | 6330 |
| RRID | AB_3670767 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8IWT1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mammalian sodium channels are heterotrimers, composed of a central, pore-forming alpha subunit and two auxiliary beta subunits (PMID: 11486343). SCN4B (sodium channel subunit beta-4) is one of several sodium channel beta subunits, which modulate channel gating kinetics. SCN4B has an N-terminal signal peptide, a large extracellular domain predicted to form an Ig-like fold, a single transmembrane alpha helix, and a short cytoplasmic C terminus. Defects in SCN4B gene are a cause of long QT syndrome type 10 (LQT10).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SCN4B antibody 83027-4-RR | Download protocol |
| IHC protocol for SCN4B antibody 83027-4-RR | Download protocol |
| FC protocol for SCN4B antibody 83027-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |