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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using SCN4B Polyclonal antibody (55438-1-AP)

    WB analysis of mouse heart using 55438-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 55438-1-AP (SCN4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SCN4B Polyclonal antibody (55438-1-AP)

    WB analysis of mouse brain using 55438-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55438-1-AP (SCN4B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using SCN4B Polyclonal antibody (55438-1-AP)

    IHC staining of human skeletal muscle using 55438-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55438-1-AP (SCN4B Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using SCN4B Polyclonal antibody (55438-1-AP)

    IHC staining of human skeletal muscle using 55438-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55438-1-AP (SCN4B Antibody) at dilution of 1:50 (under 40x lens).

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SCN4B Polyclonal antibody

SCN4B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55438-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

SCN4B, Sodium channel subunit beta 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, mouse brain tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55438-1-AP targets SCN4B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name sodium channel, voltage-gated, type IV, beta
Calculated molecular weight 25 kDa
Observed molecular weight 28 kDa
GenBank accession numberNM_174934
Gene Symbol SCN4B
Gene ID (NCBI) 6330
RRIDAB_2881331
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWT1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Mammalian sodium channels are heterotrimers, composed of a central, pore-forming alpha subunit and two auxiliary beta subunits (PMID: 11486343). SCN4B (sodium channel subunit beta-4) is one of several sodium channel beta subunits, which modulate channel gating kinetics. SCN4B has an N-terminal signal peptide, a large extracellular domain predicted to form an Ig-like fold, a single transmembrane alpha helix, and a short cytoplasmic C terminus. Defects in SCN4B gene are a cause of long QT syndrome type 10 (LQT10).

Protocols

Product Specific Protocols
WB protocol for SCN4B antibody 55438-1-APDownload protocol
IHC protocol for SCN4B antibody 55438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols