Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue, PC-12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28079-1-AP targets SCN1A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25942 Product name: Recombinant human SCN1A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 456-522 aa of NM_001165963 Sequence: MEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQK 相同性解析による交差性が予測される生物種 |
| Full Name | sodium channel, voltage-gated, type I, alpha subunit |
| Calculated molecular weight | 229 kDa |
| Observed molecular weight | 229-240 kDa |
| GenBank accession number | NM_001165963 |
| Gene Symbol | SCN1A |
| Gene ID (NCBI) | 6323 |
| RRID | AB_2881054 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35498 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sodium channel protein type 1 subunit alpha (SCN1A) is a multi-pass membrane protein that is crucial for generating and transmitting electrical signals in neurons, thereby contributing to the sensory perception of mechanically-induced pain (PMID: 14672992). SCN1A also regulates neuronal excitability and is essential for normal brain function (PMID: 37139072; 20831750).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SCN1A antibody 28079-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |