Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse brain tissue, rat Ischiadic nerve tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
55334-1-AP targets SCN10A/Nav1.8 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | sodium channel, voltage-gated, type X, alpha subunit |
| Calculated molecular weight | 221 kDa |
| GenBank accession number | NM_006514 |
| Gene Symbol | SCN10A |
| Gene ID (NCBI) | 6336 |
| RRID | AB_2881309 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y5Y9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sodium channel protein type 10 subunit alpha (SCN10A, also known as Nav1.8) is predominantly expressed in neurons of the dorsal root ganglion (DRG) and trigeminal ganglion (PMID: 8538791; 9169448). Nav1.8 markedly modulates nociceptor excitability, and thereby participates in pain‑processing mechanisms (PMID: 20367448; 11495938). Furthermore, functional SCN10A/Nav1.8 has been detected in intracardiac neurons, revealing a previously unrecognized function of this neuronal sodium channel in the modulation of cardiac electrical activity (PMID: 22723301).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for SCN10A/Nav1.8 antibody 55334-1-AP | Download protocol |
| IF protocol for SCN10A/Nav1.8 antibody 55334-1-AP | Download protocol |
| IHC protocol for SCN10A/Nav1.8 antibody 55334-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |