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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCML1 Polyclonal antibody (30257-1-AP)

    WB analysis using 30257-1-AP (same clone as 30257-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30257-1-AP (SCML1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30257-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SCML1 Polyclonal antibody (30257-1-AP)

    WB analysis of HepG2 using 30257-1-AP (same clone as 30257-1-PBS)

    WB result of SCML1 antibody (30257-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCML1 transfected HepG2 cells. This data was developed using the same antibody clone with 30257-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30257-1-PBS targets SCML1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCML1 fusion protein Ag33003 相同性解析による交差性が予測される生物種
Full Name sex comb on midleg-like 1 (Drosophila)
Calculated molecular weight 37 kDa
Observed molecular weight42 kDa
GenBank accession numberBC026159
Gene Symbol SCML1
Gene ID (NCBI) 6322
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UN30
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Sex comb on midleg-like 1 (SCML1) is a meiosis-specific protein and is an essential component of the meiotic dense body. SCML1 is preferentially expressed in germ stem cells of testis, therefore likely involved in spermatogenesis.