Validation Data Gallery
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Tested Applications
| Positive WB detected in | LNCaP cells, L02 cells, HepG2 cells, K-562 cells, HSC-T6 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
67606-1-Ig targets SCLY in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.
| Tested Reactivity | Human, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SCLY fusion protein Ag28841 相同性解析による交差性が予測される生物種 |
| Full Name | selenocysteine lyase |
| Calculated molecular weight | 49 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | BC007891 |
| Gene Symbol | SCLY |
| Gene ID (NCBI) | 51540 |
| RRID | AB_2882812 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q96I15 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SCLY antibody 67606-1-Ig | Download protocol |
| IHC protocol for SCLY antibody 67606-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Struct Mol Biol PRDX6 augments selenium utilization to limit iron toxicity and ferroptosis |