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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SCFD2 Polyclonal antibody

SCFD2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13375-1-AP

Synonyms

SCFD2, STXBP1L1

Validation Data Gallery

Applications:
  • WB analysis of COLO 320 using 13375-1-AP

    WB analysis of COLO 320 using 13375-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13375-1-AP (SCFD2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of COLO 320 using 13375-1-AP

    WB analysis of COLO 320 using 13375-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13375-1-AP (SCFD2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 13375-1-AP

    WB analysis of mouse kidney using 13375-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13375-1-AP (SCFD2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IP experiment of COLO 320 using 13375-1-AP

    IP experiment of COLO 320 using 13375-1-AP

    IP result of anti-SCFD2 (IP:13375-1-AP, 4ug; Detection:13375-1-AP 1:200) with COLO 320 cells lysate 3200ug.

  • IHC staining of human colon cancer using 13375-1-AP

    IHC staining of human colon cancer using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon cancer using 13375-1-AP

    IHC staining of human colon cancer using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human colon using 13375-1-AP

    IHC staining of human colon using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon using 13375-1-AP

    IHC staining of human colon using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inCOLO 320 cells, mouse kidney tissue
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13375-1-AP targets SCFD2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCFD2 fusion protein Ag4197 相同性解析による交差性が予測される生物種
Full Name sec1 family domain containing 2
Calculated molecular weight 684 aa, 75 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC032453
Gene symbol SCFD2
Gene ID (NCBI) 152579
RRIDAB_10646473
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SCFD2 antibody 13375-1-APDownload protocol
IHC protocol for SCFD2 antibody 13375-1-APDownload protocol
IP protocol for SCFD2 antibody 13375-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols