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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCFD2 Polyclonal antibody (13375-1-AP)

    WB analysis using 13375-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13375-1-AP (SCFD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using SCFD2 Polyclonal antibody (13375-1-AP)

    IP experiment of COLO 320 using 13375-1-AP

    IP result of anti-SCFD2 (IP:13375-1-AP, 4ug; Detection:13375-1-AP 1:600) with COLO 320 cells lysate 1680 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SCFD2 Polyclonal antibody (13375-1-AP)

    IHC staining of human colon cancer using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SCFD2 Polyclonal antibody (13375-1-AP)

    IHC staining of human colon cancer using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using SCFD2 Polyclonal antibody (13375-1-AP)

    IHC staining of human colon using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13375-1-AP (SCFD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SCFD2 Polyclonal antibody (13375-1-AP)

    IHC staining of human colon cancer using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13375-1-AP (SCFD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using SCFD2 Polyclonal antibody (13375-1-AP)

    IHC staining of human colon using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using SCFD2 Polyclonal antibody (13375-1-AP)

    IHC staining of human colon using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 40x lens).

SCFD2 Polyclonal antibody

SCFD2 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 13375-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Sec1 family domain-containing protein 2, STXBP1L1, Syntaxin-binding protein 1-like 1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOLO 320 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13375-1-AP targets SCFD2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4197

Product name: Recombinant human SCFD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC032453

Sequence: MSASGVLSFTQQGWEQVLAKVKRAVVYLDAACAESLHWGCGSTRLLEAVGGPDCHLREFEPDAIGGGAKQPKAVFVLSCLLKGRTVEILRDIICRSHFQYCVVVTTVSHAVHLTANHVPAAAAAEMEGQQPVFEQLEEKLCEWMGNMNYTAEVFHVPLLLAPVAPHFALTPAFASLFPLLPQDVHLLNSARPDKRKLGSLGDVDSTTLTPELLLQIRCLVSGLSSLCEHLGVREECFAVGSLSQVIAADLANYAPAKNRKKTAAGRASVVFVDRTLDLTGAVGHHGDNLVEKIISALPQLPGHTNDVMVNMIALTALHTEEENYNVVAPGCLSQSSDTTAKALWEALLNTK

相同性解析による交差性が予測される生物種
Full Name sec1 family domain containing 2
Calculated molecular weight 684 aa, 75 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC032453
Gene Symbol SCFD2
Gene ID (NCBI) 152579
RRIDAB_10646473
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WU76
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for SCFD2 antibody 13375-1-APDownload protocol
IP protocol for SCFD2 antibody 13375-1-APDownload protocol
WB protocol for SCFD2 antibody 13375-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Biol Cell

An integrated proximity labeling and vesicle reconstitution assay identifies novel regulators of Sonic hedgehog secretion.

Authors - Ziyang Song
  • KD Validated
View Article
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