Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 12569-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12569-1-AP (SCFD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 12569-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 12569-1-AP (SCFD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 12569-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 12569-1-AP (SCFD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 12569-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12569-1-AP (SCFD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 12569-1-AP
IP result of anti-SCFD1 (IP:12569-1-AP, 3ug; Detection:12569-1-AP 1:500) with HeLa cells lysate 2150ug.
IHC staining of human endometrial cancer using 12569-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 12569-1-AP (SCFD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human endometrial cancer using 12569-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 12569-1-AP (SCFD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 12569-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12569-1-AP (SCFD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human rectal cancer using 12569-1-AP
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 12569-1-AP (SCFD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 12569-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12569-1-AP (SCFD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse small intestine using 12569-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 12569-1-AP (SCFD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human rectal cancer using 12569-1-AP
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 12569-1-AP (SCFD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human endometrial cancer using 12569-1-AP
Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12569-1-AP (SCFD1 antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HeLa using 12569-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12569-1-AP (SCFD1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

SCFD1 Polyclonal antibody

SCFD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12569-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

C14orf163, FKSG23, KIAA0917, Sec1 family domain-containing protein 1, SLY1 homolog

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, HEK-293T cells, human kidney tissue, human liver tissue, HEK-293 cells, HeLa cells, NIH/3T3 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human endometrial cancer tissue, human rectal cancer tissue, human endometrial cancer tissue, mouse small intestine tissue, human placenta tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 6 publications below
IP	See 1 publications below

Product Information

12569-1-AP targets SCFD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, bacteria
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3234 Product name: Recombinant human SCFD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 342-642 aa of BC017734 Sequence: QQELESYRAQEDEVKRLKSIMGLEGEDEGAISMLSDNTAKLTSAVSSLPELLEKKRLIDLHTNVATAVLEHIKARKLDVYFEYEEKIMSKTTLDKSLLDIISDPDAGTPEDKMRLFLIYYISTQQAPSEADLEQYKKALTDAGCNLNPLQYIKQWKAFTKMASAPASYGSTTTKPMGLLSRVMNTGSQFVMEGVKNLVLKQQNLPVTRILDNLMEMKSNPETDDYRYFDPKMLRGNDSSVPRNKNPFQEAIVFVVGGGNYIEYQNLVDYIKGKQGKHILYGCSELFNATQFIKQLSQLGQK 相同性解析による交差性が予測される生物種
Full Name	sec1 family domain containing 1
Calculated molecular weight	642 aa, 72 kDa
Observed molecular weight	66-72 kDa
GenBank accession number	BC017734
Gene Symbol	SCFD1
Gene ID (NCBI)	23256
RRID	AB_2183266
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WVM8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SCFD1, also named as C14orf163, KIAA0917, STXBP1L2, Sly1p and SLY1, belongs to the STXBP/unc-18/SEC1 family. It is a component of the syntaxin-5 SNARE complex which regulates the early secretory pathway of eukaryotic cells at the level of endoplasmic reticulum (ER) to Golgi transport.(PMID:21242315) SCFD1 plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. It is involved in vesicular transport between the endoplasmic reticulum and the Golgi.

Protocols

Product Specific Protocols
IF protocol for SCFD1 antibody 12569-1-AP	Download protocol
IHC protocol for SCFD1 antibody 12569-1-AP	Download protocol
IP protocol for SCFD1 antibody 12569-1-AP	Download protocol
WB protocol for SCFD1 antibody 12569-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP	Autophagy Acetylation of SCFD1 regulates SNARE complex formation and autophagosome-lysosome fusion. Authors - Hong Huang KD Validated View Article
human	WB	EMBO J Direct interaction between the COG complex and the SM protein, Sly1, is required for Golgi SNARE pairing. Authors - Laufman Orly O View Article
human	WB	J Cell Sci Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex. Authors - Renna Maurizio M KD Validated View Article
Bacteria	WB	Microb Pathog Formononetin alleviates Streptococcus suis infection by targeting suilysin. Authors - Guizhen Wang View Article
human	WB	Mol Biol Cell Recruitment of PI4KIIIβ to the Golgi by ACBD3 is dependent on an upstream pathway of a SNARE complex and golgins Authors - Danièle Stalder KO Validated View Article
	WB	J Appl Microbiol Silibinin attenuates Streptococcus suis serotype 2 virulence by targeting suilysin. Authors - Xue Shen View Article

Validation Data Gallery

WB analysis using 12569-1-AP

WB analysis of human kidney using 12569-1-AP

WB analysis of human liver using 12569-1-AP

WB analysis using 12569-1-AP

IP experiment of HeLa using 12569-1-AP

IHC staining of human endometrial cancer using 12569-1-AP

IHC staining of human endometrial cancer using 12569-1-AP

IHC staining of human placenta using 12569-1-AP

IHC staining of human rectal cancer using 12569-1-AP

IHC staining of mouse kidney using 12569-1-AP

IHC staining of mouse small intestine using 12569-1-AP

IHC staining of human rectal cancer using 12569-1-AP

IHC staining of human endometrial cancer using 12569-1-AP

IF Staining of HeLa using 12569-1-AP

SCFD1 Polyclonal antibody

SCFD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Acetylation of SCFD1 regulates SNARE complex formation and autophagosome-lysosome fusion.

Direct interaction between the COG complex and the SM protein, Sly1, is required for Golgi SNARE pairing.

Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex.

Formononetin alleviates Streptococcus suis infection by targeting suilysin.

Recruitment of PI4KIIIβ to the Golgi by ACBD3 is dependent on an upstream pathway of a SNARE complex and golgins

Silibinin attenuates Streptococcus suis serotype 2 virulence by targeting suilysin.

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