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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SCEL Polyclonal antibody

SCEL Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11906-1-AP

Synonyms

SCEL, sciellin

Validation Data Gallery

Applications:
  • WB analysis of human saliva using 11906-1-AP

    WB analysis of human saliva using 11906-1-AP

    human saliva were subjected to SDS PAGE followed by western blot with 11906-1-AP (SCEL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 11906-1-AP

    WB analysis of A431 using 11906-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 11906-1-AP (SCEL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 11906-1-AP

    WB analysis using 11906-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 11906-1-AP (SCEL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of human skin using 11906-1-AP

    WB analysis of human skin using 11906-1-AP

    human skin tissue were subjected to SDS PAGE followed by western blot with 11906-1-AP (SCEL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human renal cell carcinoma using 11906-1-AP

    IHC staining of human renal cell carcinoma using 11906-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11906-1-AP (SCEL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human renal cell carcinoma using 11906-1-AP

    IHC staining of human renal cell carcinoma using 11906-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11906-1-AP (SCEL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 11906-1-AP

    IF Staining of HepG2 using 11906-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 11906-1-AP (SCEL antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 11906-1-AP

    FC experiment of HeLa using 11906-1-AP

    1X10^6 HeLa cells were stained with 0.2ug SCEL antibody (11906-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inhuman saliva tissue, A431 cells, human skin tissue, mouse skin tissue
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

11906-1-AP targets SCEL in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCEL fusion protein Ag2578 相同性解析による交差性が予測される生物種
Full Name sciellin
Calculated molecular weight 668 aa, 75 kDa
Observed molecular weight 70-78 kDa
GenBank accession numberBC020726
Gene symbol SCEL
Gene ID (NCBI) 8796
RRIDAB_2183262
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SCEL antibody 11906-1-APDownload protocol
IHC protocol for SCEL antibody 11906-1-APDownload protocol
IF protocol for SCEL antibody 11906-1-APDownload protocol
FC protocol for SCEL antibody 11906-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols