Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 28678-1-AP (same clone as 28678-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 28678-1-AP (SCD antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.
WB analysis of A431 using 28678-1-AP (same clone as 28678-1-PBS)
WB result of SCD antibody (28678-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCD transfected A431 cells. This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 28678-1-AP (same clone as 28678-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 28678-1-AP (same clone as 28678-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 28678-1-AP (same clone as 28678-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 28678-1-AP (same clone as 28678-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.
IHC staining of mouse brown adipose using 28678-1-AP (same clone as 28678-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.
IHC staining of mouse brown adipose using 28678-1-AP (same clone as 28678-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 28678-1-AP (same clone as 28678-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 28678-1-AP (same clone as 28678-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 28678-1-AP (same clone as 28678-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.
IHC staining of rat heart using 28678-1-AP (same clone as 28678-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 28678-1-AP (same clone as 28678-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SCD antibody (28678-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28678-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

SCD1 Polyclonal antibody, PBS Only

SCD1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 28678-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SCD, EC:1.14.19.1, Delta-9 desaturase, Delta(9)-desaturase, Delta(9) desaturase

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28678-1-PBS targets SCD1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag29156 Product name: Recombinant human SCD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-72 aa of BC005807 Sequence: MPAHLLQDDISSSYTTTTTITAPPSRVLQNGGDKLETMPLYLEDDIRPDIKDDIYDPTYKDKEGPSPKVEYV 相同性解析による交差性が予測される生物種
Full Name	stearoyl-CoA desaturase (delta-9-desaturase)
Calculated molecular weight	355 aa, 41 kDa
Observed molecular weight	28-42 kDa
GenBank accession number	BC005807
Gene Symbol	SCD
Gene ID (NCBI)	6319
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O00767
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

SCD1 (stearoyl-CoA desaturase) is a microsomal fatty acid monodesaturase, which catalyses the committed step in the biosynthesis of mono-unsaturated fatty acids from saturated fatty acids (PMID:10946019). SCD1 and SCD2 are the main isoforms expressed in mouse liver and brain respectively (PMID:15907797). The formation of homodimers and oligomers is an intrinsic property of SCD proteins. SCD1 is a multi-pass membrane protein and detected double bands of 37-42 kDa. The degradation product of 28 kDa may be caused by a major cleavage site at the C-terminus (PMID:15610069, PMID: 9843580).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 28678-1-AP (same clone as 28678-1-PBS)

WB analysis of A431 using 28678-1-AP (same clone as 28678-1-PBS)

IHC staining of human liver using 28678-1-AP (same clone as 28678-1-PBS)

IHC staining of human liver using 28678-1-AP (same clone as 28678-1-PBS)

IHC staining of mouse liver using 28678-1-AP (same clone as 28678-1-PBS)

IHC staining of mouse liver using 28678-1-AP (same clone as 28678-1-PBS)

IHC staining of mouse brown adipose using 28678-1-AP (same clone as 28678-1-PBS)

IHC staining of mouse brown adipose using 28678-1-AP (same clone as 28678-1-PBS)

IHC staining of human colon cancer using 28678-1-AP (same clone as 28678-1-PBS)

IHC staining of human ovary tumor using 28678-1-AP (same clone as 28678-1-PBS)

IHC staining of human stomach cancer using 28678-1-AP (same clone as 28678-1-PBS)

IHC staining of rat heart using 28678-1-AP (same clone as 28678-1-PBS)

IF Staining of MCF-7 using 28678-1-AP (same clone as 28678-1-PBS)