Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of HepG2 using 15119-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15119-1-AP (SCAMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 15119-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 15119-1-AP (SCAMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15119-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15119-1-AP (SCAMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 15119-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 15119-1-AP (SCAMP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 15119-1-AP
IP result of anti-SCAMP2 (IP:15119-1-AP, 4ug; Detection:15119-1-AP 1:500) with HepG2 cells lysate 3300 ug.
IHC staining of human pancreas cancer using 15119-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 15119-1-AP (SCAMP2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human pancreas cancer using 15119-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 15119-1-AP (SCAMP2 antibody) at dilution of 1:100 (under 10x lens).
IF Staining of HEK-293 using 15119-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SCAMP2 antibody (15119-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HEK-293 using 15119-1-AP
1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug SCAMP2 Polyclonal antibody (15119-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

SCAMP2 Polyclonal antibody

SCAMP2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 15119-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

Secretory carrier-associated membrane protein 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, HeLa cells, human liver tissue, mouse lung tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells
Positive FC (Intra) detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 1 publications below

Product Information

15119-1-AP targets SCAMP2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7190 Product name: Recombinant human SCAMP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-152 aa of BC001376 Sequence: MSAFDTNPFADPVDVNPFQDPSVTQLTNAPQGGLAEFNPFSETNAATTVPVTQLPGSSQPAVLQPSVEPTQPTPQAVVSAAQAGLLRQQEELDRKAAELERKERELQNTVANLHVRQNNWPPLPSWCPVKPCFYQDFSTEIPADYQRICKML 相同性解析による交差性が予測される生物種
Full Name	secretory carrier membrane protein 2
Calculated molecular weight	37 kDa
Observed molecular weight	39 kDa
GenBank accession number	BC001376
Gene Symbol	SCAMP2
Gene ID (NCBI)	10066
RRID	AB_2184772
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O15127
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SCAMP2 functions in post-Golgi recycling pathways. It acts as a recycling carrier to the cell surface. It couples Arf6-stimulated PLD activity to exocytosis and links this process to formation of fusion pores. SCAMP2 is marker for secretory vesicles and tobacco BY-2 cells as a model system. The MW of SCAMP2 is 36-39 kDa with modification.

Protocols

Product Specific Protocols
FC protocol for SCAMP2 antibody 15119-1-AP	Download protocol
IF protocol for SCAMP2 antibody 15119-1-AP	Download protocol
IHC protocol for SCAMP2 antibody 15119-1-AP	Download protocol
IP protocol for SCAMP2 antibody 15119-1-AP	Download protocol
WB protocol for SCAMP2 antibody 15119-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Aging (Albany NY) Expression and prognostic analyses of SCAMPs in pancreatic adenocarcinoma. Authors - Feiyu Mao View Article
human	WB,IHC	Front Immunol Identification of SCAMP2 as a regulator of NOTCH signaling in cisplatin resistance through a novel prognostic model for bladder cancer Authors - Longjun Cai View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 15119-1-AP

WB analysis of mouse lung using 15119-1-AP

WB analysis of HeLa using 15119-1-AP

WB analysis of human liver using 15119-1-AP

IP experiment of HepG2 using 15119-1-AP

IHC staining of human pancreas cancer using 15119-1-AP