Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of mouse brain using 15327-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15327-1-AP (SCAMP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 15327-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15327-1-AP (SCAMP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15327-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15327-1-AP (SCAMP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 15327-1-AP
IP result of anti-SCAMP1 (IP:15327-1-AP, 4ug; Detection:15327-1-AP 1:1000) with mouse brain tissue lysate 3200 ug.
IHC staining of mouse brain using 15327-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15327-1-AP (SCAMP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15327-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15327-1-AP (SCAMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 15327-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15327-1-AP (SCAMP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 15327-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15327-1-AP (SCAMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SCAMP1 Polyclonal antibody

SCAMP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 15327-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

SCAMP, SCAMP1, SCAMP37

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, HeLa cells, mouse pancreas tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse brain tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 2 publications below
IP	See 1 publications below

Product Information

15327-1-AP targets SCAMP1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SCAMP1 fusion protein Ag3258 相同性解析による交差性が予測される生物種
Full Name	secretory carrier membrane protein 1
Calculated molecular weight	38 kDa
Observed molecular weight	37 kDa
GenBank accession number	BC034048
Gene Symbol	SCAMP1
Gene ID (NCBI)	9522
RRID	AB_2184766
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O15126
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SCAMP1 antibody 15327-1-AP	Download protocol
IHC protocol for SCAMP1 antibody 15327-1-AP	Download protocol
IP protocol for SCAMP1 antibody 15327-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP	Int J Mol Sci Multiomics Analysis of Endocytosis upon HBV Infection and Identification of SCAMP1 as a Novel Host Restriction Factor against HBV Replication. Authors - Tanzeel Yousaf View Article
human	IHC	Cell Death Dis MTSS1 and SCAMP1 cooperate to prevent invasion in breast cancer. Authors - Jayakumar Vadakekolathu View Article
human	WB	Aging (Albany NY) Expression and prognostic analyses of SCAMPs in pancreatic adenocarcinoma. Authors - Feiyu Mao View Article
human	WB,IHC	J Cancer SCAMP1 silencing inhibits proliferation by attenuating multiple pro-survival signaling pathways in gastric cancer Authors - Gang Ma View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse brain using 15327-1-AP

WB analysis of mouse pancreas using 15327-1-AP

WB analysis of HeLa using 15327-1-AP

IP experiment of mouse brain using 15327-1-AP

IHC staining of mouse brain using 15327-1-AP

IHC staining of mouse brain using 15327-1-AP

IHC staining of human pancreas cancer using 15327-1-AP