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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SBK2 Polyclonal antibody (32117-1-AP)

    WB analysis using 32117-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32117-1-AP (SBK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using SBK2 Polyclonal antibody (32117-1-AP)

    IF Staining of A549 using 32117-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using SBK2 antibody (32117-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

SBK2 Polyclonal antibody

SBK2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32117-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Sugen kinase 69 (SgK069), SH3 domain-binding kinase family member 2, SGK069, Serine/threonine-protein kinase SBK2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HepG2 cells, Kasumi-1 cells
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32117-1-AP targets SBK2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SBK2 fusion protein Ag37233 相同性解析による交差性が予測される生物種
Full Name SH3-binding domain kinase family, member 2
Calculated molecular weight38 kDa
Observed molecular weight30 kDa
GenBank accession numberNM_001101401
Gene Symbol SBK2
Gene ID (NCBI) 646643
RRIDAB_3670197
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP0C263
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SH3 domain binding kinase family member 2 (SBK2, also known as SGK069), is an evolutionarily conserved putative serine/threonine protein kinase that plays a crucial role in cardiomyocyte differentiation, particularly in the formation and maintenance of sarcomeres. It is highly atrium-enriched and is muscle-specific, indicating its specialized function in cardiac muscle cells (PMID: 35587025). SBK2 may be useful for identifying and co-registering tumor borders during surgical resection (PMID: 23730413).

Protocols

Product Specific Protocols
WB protocol for SBK2 antibody 32117-1-APDownload protocol
IF protocol for SBK2 antibody 32117-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols