Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells, Ramos cells, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33515-1-AP targets SBF1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38267 Product name: Recombinant human SBF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1300-1430 aa of CCDS93186.1 Sequence: AGRDALAPPQANGGPPDPGFLRPQRAALYILGDKAQLKGVRSDPLQQWELVPIEVFEARQVKASFKKLLKACVPGCPAAEPSPASFLRSLEDSEWLIQIHKLLQVSVLVVELLDSGSSVLVGLEDGWDITT 相同性解析による交差性が予測される生物種 |
| Full Name | SET binding factor 1 |
| Calculated molecular weight | 208kDa，1868aa |
| Observed molecular weight | 210 kDa, 140 kDa |
| GenBank accession number | CCDS93186.1 |
| Gene Symbol | SBF1 |
| Gene ID (NCBI) | 6305 |
| RRID | AB_3742998 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O95248 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sbf1, a pseudophosphatase of the myotubularin family, is expressed at high levels in seminiferous tubules of the testis, specifically in Sertoli's cells, spermatogonia, and pachytene spermatocytes, but not in postmeiotic round spermatids. Mice that are nullizygous for Sbf1 exhibit male infertility characterized by azoospermia. Faint bands in some lanes around 150 kDa represent apparent Sbf1 degradation products (PMID: 11994405).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SBF1 antibody 33515-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |